Construct: ORF TRCN0000473685
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011356.1_s317c1
- Derived from:
- ccsbBroadEn_03979
- DNA Barcode:
- CTTAATCATGCAGCTAGGAGGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AIDA (64853)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473685
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64853 | AIDA | axin interactor, dorsalizat... | NM_022831.4 | 100% | 100% | |
| 2 | human | 64853 | AIDA | axin interactor, dorsalizat... | XR_921909.3 | 28.1% | 1_578del;930_961del;1529_3265del | |
| 3 | mouse | 108909 | Aida | axin interactor, dorsalizat... | NM_181732.4 | 93.5% | 97.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 984
- ORF length:
- 918
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggaggtgacc cggagtctgc tgcagcgctg gggcgccagt tttaggagag 121 gcgccgactt cgactcttgg ggccagctgg tggaggcgat agacgagtat cagatattag 181 caagacatct acaaaaggag gcccaagctc aacacaataa ttctgaattc acagaagaac 241 aaaagaaaac cataggcaaa attgcaacat gcttggaatt gcgaagtgca gctttacagt 301 ccacacagtc tcaagaagaa tttaaactgg aggacctgaa gaagctagaa ccaatcctaa 361 agaatattct tacatataat aaagaattcc catttgatgt tcagcctgtc ccattaagaa 421 gaattttggc acctggtgaa gaagagaatt tggaatttga agaagatgaa gaagagggtg 481 gtgctggagc agggtctcct gattcttttc ctgctagagt tcccggtact ttattaccaa 541 ggttgccatc ggaaccagga atgacattac tcactatcag aattgagaaA ATTGGTTTGA 601 AAGATGCTGG GCAGTGCATC GATCCCTATA TTACAGTTAG TGTAAAGGAT CTGAATGGCA 661 TAGACTTAAC TCCTGTGCAA GATACTCCTG TGGCTTCAAG AAAAGAAGAT ACATATGTTC 721 ATTTTAATGT GGACATTGAG CTCCAGAAGC ATGTTGAAAA ATTAACCAAA GGTGCAGCTA 781 TCTTCTTTGA ATTCAAACAC TACAAGCCTA AAAAAAGGTT TACCAGCACC AAGTGTTTTG 841 CTTTCATGGA GATGGATGAA ATTAAACCTG GGCCAATTGT AATAGAACTA TACAAGAAAC 901 CCACTGACTT TAAAAGAAAG AAATTGCAAT TATTGACCAA GAAACCACTT TATCTTCATC 961 TACATCAAAC TTTGCACAAG GAATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1021 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1081 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TTAATCATGC 1141 AGCTAGGAGG GGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1201 att