Construct: ORF TRCN0000473686
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010436.1_s317c1
- Derived from:
- ccsbBroadEn_01116
- DNA Barcode:
- ATCTCCGAATAAGACCCTTATAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OGN (4969)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473686
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4969 | OGN | osteoglycin | NM_014057.5 | 100% | 100% | |
| 2 | human | 4969 | OGN | osteoglycin | NM_033014.3 | 100% | 100% | |
| 3 | human | 4969 | OGN | osteoglycin | NM_024416.4 | 83.4% | 83.1% | 1_174del;176_177delCAinsTG;179C>A |
| 4 | mouse | 18295 | Ogn | osteoglycin | NM_008760.4 | 88.8% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gactctgcag tctacacttc tcctgttact gcttgtgcct ctgataaagc 121 cagcaccacc aacccagcag gactcacgca ttatctatga ttatggaaca gataattttg 181 aagaatccat atttagccaa gattatgagg ataaatacct ggatggaaaa aatattaagg 241 aaaaagaaac tgtgataata cccaatgaga aaagtcttca attacaaaaa gatgaggcaa 301 taacaccatt acctcccaag aaagaaaatg atgaaatgcc cacgtgtctg ctgtgtgttt 361 gtttaagtgg ctctgtatac tgtgaagaag ttgacattga tgctgtacca cccttaccaa 421 aggaatcagc ctatctttac gcaCGATTCA ACAAAATTAA AAAGCTGACT GCCAAAGATT 481 TTGCAGACAT ACCTAACTTA AGAAGACTCG ATTTTACAGG AAATTTGATA GAAGATATAG 541 AAGATGGTAC TTTTTCAAAA CTTTCTCTGT TAGAAGAACT TTCACTTGCT GAAAATCAAC 601 TACTAAAACT TCCAGTTCTT CCTCCCAAGC TCACTTTATT TAATGCAAAA TACAACAAAA 661 TCAAGAGTAG GGGAATCAAA GCAAATGCAT TCAAAAAACT GAATAACCTC ACCTTCCTCT 721 ACTTGGACCA TAATGCCCTG GAATCCGTGC CTCTTAATTT ACCAGAAAGT CTACGTGTAA 781 TTCATCTTCA GTTCAACAAC ATAGCTTCAA TTACAGATGA CACATTCTGC AAGGCTAATG 841 ACACCAGTTA CATCCGGGAC CGCATTGAAG AGATACGCCT GGAGGGCAAT CCAATCGTCC 901 TGGGAAAGCA TCCAAACAGT TTTATTTGCT TAAAAAGATT ACCGATAGGG TCATACTTTT 961 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGAATCTC CGAATAAGAC CCTTATAACA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt