Construct: ORF TRCN0000473697
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014641.1_s317c1
- Derived from:
- ccsbBroadEn_09432
- DNA Barcode:
- TTTATTGACAGCAGCCAGGTGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACSM1 (116285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473697
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | NM_001318890.1 | 99.8% | 100% | 1362T>A;1458C>T;1647G>A |
| 2 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | NM_052956.3 | 99.8% | 100% | 1362T>A;1458C>T;1647G>A |
| 3 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XM_006721016.3 | 93.9% | 94.1% | (many diffs) |
| 4 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XM_006721017.3 | 89.2% | 89.4% | (many diffs) |
| 5 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XM_011545729.3 | 78.5% | 78.5% | 1361_1362delCCins371 |
| 6 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XR_950737.3 | 77.5% | (many diffs) | |
| 7 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XR_243258.3 | 72.9% | (many diffs) | |
| 8 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XR_950738.3 | 72.7% | (many diffs) | |
| 9 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XM_011545730.3 | 72.5% | 70.7% | 990_991ins307;1050_1051ins167;1173G>A |
| 10 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XR_001751827.2 | 68.1% | (many diffs) | |
| 11 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XR_429672.3 | 66.2% | (many diffs) | |
| 12 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XM_011545731.2 | 64.8% | 64.4% | (many diffs) |
| 13 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | NR_134918.1 | 63.3% | (many diffs) | |
| 14 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XR_001751828.2 | 63.1% | (many diffs) | |
| 15 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XM_006721018.2 | 61.6% | 61.8% | (many diffs) |
| 16 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XM_011545732.2 | 61.6% | 61.8% | (many diffs) |
| 17 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XM_017022914.2 | 61.2% | 57.5% | (many diffs) |
| 18 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XM_017022915.1 | 58.2% | 57.3% | (many diffs) |
| 19 | human | 116285 | ACSM1 | acyl-CoA synthetase medium ... | XM_024450148.1 | 55.8% | 55.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1800
- ORF length:
- 1731
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagtggcta atgaggttcc ggaccctctg gggcatccac aaatccttcc 121 acaacatcca ccctgcccct tcacagctgc gctgccggtc tttatcagaa tttggagccc 181 caagatggaa tgactatgaa gtaccggagg aatttaactt tgcaagttat gtactggact 241 actgggctca aaaggagaag gagggcaaga gaggtccaaa tccagctttt tggtgggtga 301 atggccaagg ggatgaagta aagtggagct tcagagagat gggagaccta acccgccgtg 361 tagccaacgt cttcacacag acctgtggcc tacaacaggg agaccatctg gccttgatgc 421 tgcctcgagt tcctgagtgg tggctggtgg ctgtgggctg catgcgaaca gggatcatct 481 tcattcctgc gaccatcctg ttgaaggcca aagacattct ctatcgacta cagttgtcta 541 aagccaaggg cattgtgacc atagatgccc ttgcctcaga ggtggactcc atagcttctc 601 agtgcccctc tctgaaaacc aagctcctgg tgtctgatca cagccgtgaa gggtggctgg 661 acttccgatc gctggttaaa tcagcatccc cagaacacac ctgtgttaag tcaaagacct 721 tggacccaat ggtcatcttc ttcaccagtg ggaccacagg cttccccaag atggcaaaac 781 actcccatgg gttggcctta caaccctcct tcccaggaag taggaaatta cggagcctga 841 agacatctga tgtctcctgg tgcctgtcgg actcaggatg gattgtggct accatttgga 901 ccctggtaga accatggaca gcgggttgta cagtctttat ccaccatctg ccacagtttg 961 acaccaaggt catcatacag acattgttga aataccccat taaccacttt tggggggtat 1021 catctatata tcgaatgatt ctgcagcagg atttcaccag catcaggttc cctgccctgg 1081 agcactgcta tactggcggg gaggtcgtgt tgcccaagga tcaggaggag tggaaaagac 1141 ggacgggcct tctgctctac gagaactatg ggcagtcgga aacgggacta atttgtgcca 1201 cctactgggg aatgaagatc aagccgggtt tcatggggaa ggccactcca ccctacgacg 1261 tccaggtcat tgatgacaag ggcagcatcc tgccaccTAA CACAGAAGGA AACATTGGCA 1321 TCAGAATCAA ACCTGTCAGG CCTGTGAGCC TCTTCATGTG CTATGAGGGT GACCCAGAGA 1381 AGACAGCTAA AGTGGAATGT GGGGACTTCT ACAACACTGG GGACAGAGGA AAGATGGATG 1441 AAGAGGGCTA CATTTGTTTC CTGGGGAGGA GTGATGACAT CATTAATGCC TCTGGGTATC 1501 GCATCGGGCC TGCAGAGGTT GAAAGTGCTT TGGTGGAGCA CCCAGCGGTG GCGGAGTCAG 1561 CCGTGGTGGG CAGCCCAGAC CCGATTCGAG GGGAGGTGGT GAAGGCCTTT ATTGTCCTGA 1621 CCCCACAGTT CCTGTCCCAT GACAAGGATC AGCTGACCAA GGAACTGCAG CAGCATGTCA 1681 AGTCAGTGAC AGCCCCATAC AAGTACCCAA GGAAAGTGGA GTTTGTCTCA GAGCTGCCAA 1741 AAACCATCAC TGGCAAGATT GAACGGAAGG AACTTCGGAA AAAGGAGACT GGTCAGATGT 1801 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1861 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1921 TTTATATATC TTGTGGAAAG GACGATTTAT TGACAGCAGC CAGGTGCTCA CGCGTTAAGT 1981 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt