Construct: ORF TRCN0000473719
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011475.1_s317c1
- Derived from:
- ccsbBroadEn_12292
- DNA Barcode:
- ACACTCACATTATGGGGCCACTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SULF2 (55959)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473719
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55959 | SULF2 | sulfatase 2 | NM_001161841.1 | 17.1% | 17.1% | 1_2163del |
| 2 | human | 55959 | SULF2 | sulfatase 2 | NM_018837.3 | 17.1% | 17.1% | 1_2163del |
| 3 | human | 55959 | SULF2 | sulfatase 2 | XM_005260458.3 | 17.1% | 17.1% | 1_2163del |
| 4 | human | 55959 | SULF2 | sulfatase 2 | XM_006723830.2 | 17.1% | 17.1% | 1_2163del |
| 5 | human | 55959 | SULF2 | sulfatase 2 | XM_017027955.2 | 17.1% | 17.1% | 1_2163del |
| 6 | human | 55959 | SULF2 | sulfatase 2 | XM_006723827.2 | 17.1% | 17.1% | 1_2166del |
| 7 | human | 55959 | SULF2 | sulfatase 2 | XM_006723828.2 | 17.1% | 17.1% | 1_2166del |
| 8 | human | 55959 | SULF2 | sulfatase 2 | XM_006723829.3 | 17.1% | 17.1% | 1_2166del |
| 9 | human | 55959 | SULF2 | sulfatase 2 | XM_011528913.1 | 17.1% | 17.1% | 1_2166del |
| 10 | human | 55959 | SULF2 | sulfatase 2 | XM_011528914.2 | 17.1% | 17.1% | 1_2166del |
| 11 | human | 55959 | SULF2 | sulfatase 2 | NM_198596.2 | 16.7% | 16.7% | 1_2163del;2493_2494insGGACTTAAA |
| 12 | human | 55959 | SULF2 | sulfatase 2 | XM_011528916.2 | 15% | 15% | 1_2163del;2528_2529ins54 |
| 13 | human | 55959 | SULF2 | sulfatase 2 | XM_011528915.2 | 15% | 15% | 1_2166del;2531_2532ins54 |
| 14 | mouse | 72043 | Sulf2 | sulfatase 2 | NM_001252578.1 | 15.3% | 16.3% | (many diffs) |
| 15 | mouse | 72043 | Sulf2 | sulfatase 2 | NM_028072.5 | 15.3% | 16.3% | (many diffs) |
| 16 | mouse | 72043 | Sulf2 | sulfatase 2 | XM_006500209.3 | 15.3% | 16.3% | (many diffs) |
| 17 | mouse | 72043 | Sulf2 | sulfatase 2 | XM_006500211.2 | 15.3% | 16.3% | (many diffs) |
| 18 | mouse | 72043 | Sulf2 | sulfatase 2 | XM_017319268.1 | 15.3% | 16.3% | (many diffs) |
| 19 | mouse | 72043 | Sulf2 | sulfatase 2 | NM_001252579.1 | 14.8% | 15.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 513
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc aggcctcacg tgcttcaccc acgacaacca gcactggcag acggcgcctt 121 tctggacact ggggcctttc tgtgcctgca ccagcgccaa CAATAACACG TACTGGTGCA 181 TGAGGACCAT CAATGAGACT CACAATTTCC TCTTCTGTGA ATTTGCAACT GGCTTCCTAG 241 AGTACTTTGA TCTCAACACA GACCCCTACC AGCTGATGAA TGCAGTGAAC ACACTGGACA 301 GGGATGTCCT CAACCAGCTA CACGTACAGC TCATGGAGCT GAGGAGCTGC AAGGGTTACA 361 AGCAGTGTAA CCCCCGGACT CGAAACATGG ACCTGGGACT TAAAGATGGA GGAAGCTATG 421 AGCAATACAG GCAGTTTCAG CGTCGAAAGT GGCCAGAAAT GAAGAGACCT TCTTCCAAAT 481 CACTGGGACA ACTGTGGGAA GGCTGGGAAG GTTACCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 661 ACTCACATTA TGGGGCCACT ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt