Construct: ORF TRCN0000473723

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002411.1_s317c1
Derived from:
ccsbBroadEn_00431
DNA Barcode:
CATCCTTGGTCAACCAGGTCATTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX6 (1656)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473723
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1656 DDX6 DEAD-box helicase 6 NM_001257191.2 100% 100%
2 human 1656 DDX6 DEAD-box helicase 6 NM_004397.6 100% 100%
3 human 1656 DDX6 DEAD-box helicase 6 XM_005271417.4 100% 100%
4 human 1656 DDX6 DEAD-box helicase 6 XM_024448377.1 100% 100%
5 human 1656 DDX6 DEAD-box helicase 6 XM_011542644.1 91% 91% 863_864ins129
6 human 1656 DDX6 DEAD-box helicase 6 XM_017017251.1 91% 91% 863_864ins129
7 human 1656 DDX6 DEAD-box helicase 6 XM_011542645.1 89.8% 89.8% 497_498ins147
8 mouse 13209 Ddx6 DEAD (Asp-Glu-Ala-Asp) box ... NM_001110826.1 93.5% 97.7% (many diffs)
9 mouse 13209 Ddx6 DEAD (Asp-Glu-Ala-Asp) box ... NM_007841.4 93.5% 97.7% (many diffs)
10 mouse 13209 Ddx6 DEAD (Asp-Glu-Ala-Asp) box ... NM_181324.3 93.5% 97.7% (many diffs)
11 mouse 13209 Ddx6 DEAD (Asp-Glu-Ala-Asp) box ... XM_011242391.2 93.5% 97.7% (many diffs)
12 mouse 13209 Ddx6 DEAD (Asp-Glu-Ala-Asp) box ... XM_011242392.2 93.5% 97.7% (many diffs)
13 mouse 13209 Ddx6 DEAD (Asp-Glu-Ala-Asp) box ... XM_017313124.1 93.5% 97.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1515
ORF length:
1449
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag cacggccaga acagagaacc ctgttataat gggtctgtcc agtcaaaatg
121gtcagctgag aggccctgtg aaacccactg gtggccctgg aggagggggc acacagacac
181agcaacagat gaaccagctg aaaaacacca acacaatcaa taatggcact cagcagcaag
241cacagagtat gaccaccact attaaacctg gtgatgactg gaaaaagact ttaaaactcc
301ctccaaagga tctaagaatc aaaacttcgg atgtgacctc cacaaaagga aatgagtttg
361aagattactg tttgaaacgg gagttactga tgggaatttt tgaaatgggc tgggaaaagc
421catctcctat tcaggaggag agcattccca ttgctttatc tggtagggat atcttagcta
481gagcaaaaaa tggaacaggc aagagcggtg cctacctcat tcccttactt gaacggctag
541acctgaagaa ggacaatata caagcaatgg tgattgttcc cactagagaa cttgctctac
601aggtcagtca aatttgcatc caggtcagca aacacatggg aggggccaaa gtgatggcaa
661ccacaggagg aaccaattta cgagatgaca taatgaggct tgatgataca gtgcacgtgg
721tgattgctac ccctgggaga atcctggatc ttattaagaa aggagtagca aaggttgatc
781atgtccagat gatagtattg gatgaggcag ataagttgct gtcacaggat tttgtgcaga
841taatggagga tattattctc acgctaccta aaaacaggca gattttacta tattccgcta
901ctttccctct tagtgtacag aagttcatga attcccattt gcagaaaccc tatgagatta
961acctgatgga ggaactaact ctgaagggag taacccagta ctacgcatat gtaactgagc
1021gccaaaaagt acactgcctc aacacacttt tctccaggct tcagataaac cagtcgatca
1081ttttctgtaa ctcctcTCAG CGAGTTGAAT TGCTAGCCAA GAAGATTTCT CAACTGGGTT
1141ATTCTTGCTT CTATATTCAT GCTAAAATGA GGCAGGAACA TCGAAATCGT GTATTTCATG
1201ATTTCCGAAA TGGCTTATGC CGCAATCTTG TTTGCACTGA TCTGTTTACC CGAGGTATTG
1261ATATACAAGC TGTGAATGTG GTAATAAACT TTGATTTCCC AAAGCTGGCA GAGACCTATC
1321TCCATCGTAT TGGAAGATCA GGTCGCTTTG GTCATCTTGG CTTAGCCATC AACTTGATCA
1381CATATGATGA TCGCTTCAAC CTGAAAAGTA TTGAGGAGCA GCTGGGAACA GAAATTAAAC
1441CTATTCCGAG CAACATTGAT AAGAGCCTGT ATGTGGCAGA ATACCACAGC GAGCCTGTAG
1501AAGATGAGAA ACCTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1561CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1621TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CATCCTTGGT CAACCAGGTC
1681ATTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)