Construct: ORF TRCN0000473729
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014114.1_s317c1
- Derived from:
- ccsbBroadEn_10755
- DNA Barcode:
- AATCACGCGGTTTTTAGATCGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VCAN (1462)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473729
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1462 | VCAN | versican | NM_001126336.3 | 53.6% | 53.4% | (many diffs) |
| 2 | human | 1462 | VCAN | versican | NM_001164098.1 | 21.5% | 21.1% | (many diffs) |
| 3 | human | 1462 | VCAN | versican | NM_001164097.1 | 14.6% | 14.4% | (many diffs) |
| 4 | human | 1462 | VCAN | versican | NM_004385.5 | 10.4% | 10.2% | (many diffs) |
| 5 | mouse | 13003 | Vcan | versican | NM_172955.1 | 83.9% | 84.7% | (many diffs) |
| 6 | mouse | 13003 | Vcan | versican | NM_001134475.1 | 46.2% | 47.1% | (many diffs) |
| 7 | mouse | 13003 | Vcan | versican | NM_001134474.1 | 18.9% | 19.1% | (many diffs) |
| 8 | mouse | 13003 | Vcan | versican | NM_019389.2 | 12.8% | 12.8% | (many diffs) |
| 9 | mouse | 13003 | Vcan | versican | XM_011244470.1 | 9.4% | 9.4% | (many diffs) |
| 10 | mouse | 13003 | Vcan | versican | NM_001081249.1 | 9.1% | 9.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cataaatata aagagcatct tatggatgtg ttcaacctta atagtaaccc 121 atgcgctaca taaagtcaaa gtgggaaaaa gcccaccggt gaggggctcc ctctctggaa 181 aagtcagcct accttgtcat ttttcaacga tgcctacttt gccacccagt tacaacacca 241 gtgaatttct ccgcatcaaa tggtctaaga ttgaagtgga caaaaatgga aaagatttga 301 aagagactac tgtccttgtg gcccaaaatg gaaatatcaa gattggtcag gactacaaag 361 ggagagtgtc tgtgcccaca catcccgagg ctgtgggcga tgcctccctc actgtggtca 421 agctgctggc aagtgatgcg ggtctttacc gctgtgacgt catgtacggg attgaagaca 481 cacaagacac ggtgtcactg actgtggatg gggttgtgtt tcactacagg gcggcaacca 541 gcaggtacac actgaatttt gaggctgctc agaaggcttg tttggacgtt ggggcagtca 601 tagcaactcc agagcagctc tttgctgcct atgaagatgg atttgagcag tgtgacgcag 661 gctggctggc tgatcagact gTCAGATATC CCATCCGGGC TCCCAGAGTA GGCTGTTATG 721 GAGATAAGAT GGGAAAGGCA GGAGTCAGGA CTTATGGATT CCGTTCTCCC CAGGAAACTT 781 ACGATGTGTA TTGTTATGTG GATCATCTGG ATGGTGATGT GTTCCACCTC ACTGTCCCCA 841 GTAAATTCAC CTTCGAGGAG GCTGCAAAAG AGTGTGAAAA CCAGGATGCC AGGCTGGCAA 901 CAGTGGGGGA ACTCCAGGCG GCATGGAGGA ACGGCTTTGA CCAGTGCGAT TACGGGTGGC 961 TGTCGGATGC CAGCGTGCGC CACCCTGTGA CTGTGGCCAG GGCCCAGTGT GGAGGTGGTC 1021 TACTTGGGGT GAGAACCCTG TATCGTTTTG AGAACCAGAC AGGCTTCCCT CCCCCTGATA 1081 GCAGATTTGA TGCCTACTGC TTTAAACGTA AGTGTTTGAT ACCTTTTTAC CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGAAATCACG CGGTTTTTAG ATCGTTAACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt