Transcript: Human NM_001164098.1

Homo sapiens versican (VCAN), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
VCAN (1462)
Length:
7154
CDS:
357..5285

Additional Resources:

NCBI RefSeq record:
NM_001164098.1
NBCI Gene record:
VCAN (1462)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001164098.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000323355 ACTCGCCTTGAAGCGACTATT pLKO_005 3384 CDS 100% 13.200 18.480 N VCAN n/a
2 TRCN0000033634 CCGATATTGATAGAGAGTATT pLKO.1 4174 CDS 100% 13.200 18.480 N VCAN n/a
3 TRCN0000344029 TTTGAGTATCCTCGCAGAAAC pLKO_005 1421 CDS 100% 10.800 15.120 N VCAN n/a
4 TRCN0000033638 CCGCATCAAATGGTCTAAGAT pLKO.1 542 CDS 100% 5.625 7.875 N VCAN n/a
5 TRCN0000323274 ACAATGAGTTTGGGCATATTT pLKO_005 5674 3UTR 100% 15.000 10.500 N VCAN n/a
6 TRCN0000350815 GATTTAGGCTCAGGATTATTT pLKO_005 3891 CDS 100% 15.000 10.500 N VCAN n/a
7 TRCN0000344092 GGGAATTTCCACAGTTATTTA pLKO_005 2354 CDS 100% 15.000 10.500 N VCAN n/a
8 TRCN0000323353 ATGGATGTGTTCAACCTTAAT pLKO_005 383 CDS 100% 13.200 9.240 N VCAN n/a
9 TRCN0000344093 GTGAATTTCTCCGCATCAAAT pLKO_005 532 CDS 100% 13.200 9.240 N VCAN n/a
10 TRCN0000033637 GCACCTGTTATCCTACTGAAA pLKO.1 4399 CDS 100% 4.950 3.465 N VCAN n/a
11 TRCN0000033635 GCCAACTACATCAACTGGTAT pLKO.1 3008 CDS 100% 4.950 3.465 N VCAN n/a
12 TRCN0000344091 CAATGAGTTTGGGCATATTTA pLKO_005 5675 3UTR 100% 15.000 9.000 N VCAN n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001164098.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10755 pDONR223 100% 21.5% 21.1% None (many diffs) n/a
2 ccsbBroad304_10755 pLX_304 0% 21.5% 21.1% V5 (many diffs) n/a
3 TRCN0000473729 AATCACGCGGTTTTTAGATCGTTA pLX_317 42.2% 21.5% 21.1% V5 (many diffs) n/a
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