Construct: ORF TRCN0000473738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009929.1_s317c1
- Derived from:
- ccsbBroadEn_02312
- DNA Barcode:
- CGAGTGATCCCACTACCCTTACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARPC1B (10095)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473738
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10095 | ARPC1B | actin related protein 2/3 c... | NM_005720.4 | 100% | 100% | |
| 2 | human | 10095 | ARPC1B | actin related protein 2/3 c... | XM_006715825.2 | 100% | 100% | |
| 3 | human | 10095 | ARPC1B | actin related protein 2/3 c... | XM_006715826.2 | 100% | 100% | |
| 4 | human | 10095 | ARPC1B | actin related protein 2/3 c... | XM_024446628.1 | 100% | 100% | |
| 5 | human | 10095 | ARPC1B | actin related protein 2/3 c... | XM_024446629.1 | 100% | 100% | |
| 6 | mouse | 11867 | Arpc1b | actin related protein 2/3 c... | NM_023142.2 | 88.7% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1182
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctaccacagc ttcctggtgg agcccatcag ctgccacgcc tggaacaagg 121 accgcaccca gattgccatc tgccccaaca accatgaggt gcatatctat gaaaagagcg 181 gtgccaaatg gaccaaggtg cacgagctca aggagcacaa cgggcaggtg acaggcatcg 241 actgggcccc cgagagtaac cgtattgtga cctgcggcac agaccgcaac gcctacgtgt 301 ggacgctgaa gggccgcaca tggaagccca cgctggtcat cctgcggatc aaccgggctg 361 cccgctgcgt gcgctgggcc cccaacgaga acaagtttgc tgtgggcagc ggctctcgtg 421 tgatctccat ctgttatttc gagcaggaga atgactggtg ggtttgcaag cacatcaaga 481 agcccatccg ctccaccgtc ctcagcctgg actggcaccc caacaatgtg ctgctggctg 541 ccggctcctg tgacttcaag tgtcggatct tttcagccta catcaaggag gtggaggaac 601 ggccggcacc caccccgtgg ggctccaaga tgccctttgg ggaactgatg ttcgaatcca 661 gcagtagctg cggctgggta catggcgtct gtttctcagc cagcgggagc cgcgtggcct 721 gggtaagcca cgacagcacc gTCTGCCTGG CTGATGCCGA CAAGAAGATG GCCGTCGCGA 781 CTCTGGCCTC TGAAACACTA CCACTGCTGG CGCTGACCTT CATCACAGAC AACAGCCTGG 841 TGGCAGCGGG CCACGACTGC TTCCCGGTGC TGTTCACCTA TGACGCCGCC GCGGGGATGC 901 TGAGCTTCGG CGGGCGGCTG GACGTTCCTA AGCAGAGCTC GCAGCGTGGC TTGACGGCCC 961 GCGAGCGCTT CCAGAACCTG GACAAGAAGG CGAGCTCCGA GGGTGGCACG GCTGCGGGCG 1021 CGGGCCTAGA CTCGCTGCAC AAGAACAGCG TCAGCCAGAT CTCGGTGCTC AGCGGCGGCA 1081 AGGCCAAGTG CTCGCAGTTC TGCACCACTG GCATGGATGG CGGCATGAGT ATCTGGGATG 1141 TGAAGAGCTT GGAGTCAGCC TTGAAGGACC TCAAGATCAA ATGCCCAACT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGACGA GTGATCCCAC TACCCTTACC GACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t