Construct: ORF TRCN0000473741
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003042.1_s317c1
- Derived from:
- ccsbBroadEn_16044
- DNA Barcode:
- GCCTTACCCCCCACCTGCTGAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM220A (84792)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473741
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 644596 | SMIM10L2B | small integral membrane pro... | NM_001348255.2 | 71.6% | 73.7% | (many diffs) |
| 2 | human | 399668 | SMIM10L2A | small integral membrane pro... | NM_203306.3 | 71.2% | 73.7% | (many diffs) |
| 3 | human | 100507415 | FAM220CP | family with sequence simila... | XR_002956924.1 | 7.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 320
- ORF end:
- 524
- ORF length:
- 204
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA TGGCGGCGGC TCTGTCGGGC CTGGCTGTCC GGCTCTCGCG 361 CTCGGCCGCC GCCCGCTCCT ATGGGGTCTT CTGCAAGGGG CTGACTCGCA CGCTGCTCAT 421 CTTCTTCGAC CTGGCCTGGC GGCTGCGTAT CAACTTCCCC TACCTCTACA TCGTGGCTTC 481 CATGATGCTC AACGTCCGCC TGCAGGTTCA TATTGAGATC CATTGCCCAA CTTTCTTGTA 541 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 601 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 661 AAAGGACGAG CCTTACCCCC CACCTGCTGA ATAACGCGTT AAGTCgacaa tcaacctctg 721 gattacaaaa tttgtgaaag att