Construct: ORF TRCN0000473759

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009433.1_s317c1
Derived from:
ccsbBroadEn_09841
DNA Barcode:
CGTAGATGACCACACTACTATCTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CABCOCO1 (219621)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473759
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 219621 CABCOCO1 ciliary associated calcium ... NM_001366905.1 99.8% 100% 489T>C
2 human 219621 CABCOCO1 ciliary associated calcium ... NM_173554.2 99.8% 100% 489T>C
3 human 219621 CABCOCO1 ciliary associated calcium ... NM_001366908.1 83% 83.2% 1_126del;615T>C
4 human 219621 CABCOCO1 ciliary associated calcium ... XM_005269598.3 83% 83.2% 1_126del;615T>C
5 human 219621 CABCOCO1 ciliary associated calcium ... NM_001366906.1 70.1% 70.2% 1_264del;753T>C
6 human 219621 CABCOCO1 ciliary associated calcium ... NM_001366909.1 49.3% 46.1% (many diffs)
7 human 219621 CABCOCO1 ciliary associated calcium ... NM_001366907.1 34.7% 32.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
690
ORF length:
624
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga tttctctatt attcagtatt caaaatttat gactttacta gctatgtcac
121ttcaaaatct taaaacacta catatgtcct taGAGGAAAG CATAAAATGG CTTGGAGAAG
181TTATGGCTGA AATAGGACCA ACACATTCGC AAAAGAGTGA GGACTGGAAT ATCTTTGATG
241TAAAACAAGC CAATGCTATC ATTGATTACT TAAAAATCAG CTTATTTCAA CACTACAAGC
301TATACGAGTT TATGTTCTAC TCTGCCAGGG AAGAAATTGT GATAGGAACT GAGCAAGTGA
361TAGAGGTTGT CAAGTCTGCA TGTGGCCCTT TCCCAAATCC TCTGGAAGAA GGAATCTCAT
421TTGATATTTA TTCAACATTC ATAGAGCCCC CCACAATATT GGATACGGAA ATGAAGAGGT
481TGGATCAAGA ACAAGGCCCT GAGGAGTCTC AGCCAGAAAC TGACACCTCA GACATGGATC
541CTTTAGTTGG TTTCACCATT GAAGATGTAA AATCAGTGCT GGATCAAGTC ACAGATGACA
601TTTTAATCGG CATTCAGACC GAGATAAACG AAAAACTGCA AATACAGGAA GAGGCCTTTA
661ATGCACGAAT AGAAAAATTG AAAAAGGCCT ACCCAACTTT CTTGTACAAA GTGGTTGATA
721TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
781CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGTAG
841ATGACCACAC TACTATCTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
901tgaaagatt

Download FASTA (ORF) (Full)