Construct: ORF TRCN0000473774
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006315.1_s317c1
- Derived from:
- ccsbBroadEn_14437
- DNA Barcode:
- CTGTGCGCGCGTAAGAGCCGTCGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RTKN2 (219790)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473774
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219790 | RTKN2 | rhotekin 2 | NM_001282941.2 | 99.7% | 95.7% | 468delT |
| 2 | human | 219790 | RTKN2 | rhotekin 2 | XM_017015844.2 | 32% | 30.8% | 468delT;489_1518delinsG |
| 3 | human | 219790 | RTKN2 | rhotekin 2 | XM_011539460.2 | 30.8% | 29.6% | 468delT;489_1578delinsG |
| 4 | human | 219790 | RTKN2 | rhotekin 2 | XM_017015843.1 | 30.7% | 29.5% | 468delT;489_1584delinsG |
| 5 | human | 219790 | RTKN2 | rhotekin 2 | XM_011539457.3 | 30% | 28.8% | 468delT;489_1623delinsG |
| 6 | human | 219790 | RTKN2 | rhotekin 2 | XR_945618.2 | 29.6% | 1_269del;737delT;758_1644delinsG | |
| 7 | human | 219790 | RTKN2 | rhotekin 2 | NM_145307.4 | 26.6% | 25.6% | 468delT;489_1827delinsG |
| 8 | human | 219790 | RTKN2 | rhotekin 2 | XM_011539456.2 | 25.2% | 24.2% | 468delT;489_1932delinsG |
| 9 | human | 219790 | RTKN2 | rhotekin 2 | XR_001747053.1 | 7% | 1_269del;737delT;758_6927delinsG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 543
- ORF length:
- 477
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggggccgagc ctgaggggtc ctgcgcTCCG CCTGGCGGGG CTTCCCACCC 121 AGCAGGACTG CAACATTCAA GAAAAAATAG ACTTAGAAAT TCGAATGCGA GAAGGAATAT 181 GGAAACTCCT TTCTCTGAGC ACTCAGAAAG ATCAAGTTTT ACATGCAGTT AAGAATCTCA 241 TGGTGTGCAA TGCTCGACTA ATGGCCTATA CATCGGAGCT ACAGAAATTA GAAGAACAGA 301 TTGCAAATCA GACTGGAAGA TGTGATGTGA AATTTGAAAG TAAAGAACGA ACAGCATGTA 361 AAGGAAAGAT TGCCATATCA GATATTCGAA TACCACTAAT GTGGAAAGAC TCTGATCACT 421 TCAGCAATAA AGAACGATCA CGACGCTATG CCATTTTTTG TTTATTCAAA ATGGGAGCTA 481 ATGTGTTTGA TACTGATGTG GTGAATGTGG ATAAAACAAT CACAGATATA TGTTTGAAAA 541 TGTAACCATA TTGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 601 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 661 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TGTGCGCGCG TAAGAGCCGT 721 CGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att