Construct: ORF TRCN0000473777
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000110.2_s317c1
- Derived from:
- ccsbBroadEn_03382
- DNA Barcode:
- CTCAGGTTCCCCGAGCCGGTACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K20 (51776)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473777
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51776 | MAP3K20 | mitogen-activated protein k... | NM_133646.3 | 100% | 100% | |
| 2 | human | 51776 | MAP3K20 | mitogen-activated protein k... | XM_017004323.1 | 100% | 100% | |
| 3 | human | 51776 | MAP3K20 | mitogen-activated protein k... | XM_017004324.1 | 100% | 100% | |
| 4 | human | 51776 | MAP3K20 | mitogen-activated protein k... | NM_016653.3 | 49.7% | 44.6% | (many diffs) |
| 5 | human | 51776 | MAP3K20 | mitogen-activated protein k... | XM_005246640.2 | 49.7% | 44.6% | (many diffs) |
| 6 | mouse | 65964 | Map3k20 | mitogen-activated protein k... | NM_001164791.1 | 93.1% | 96.4% | (many diffs) |
| 7 | mouse | 65964 | Map3k20 | mitogen-activated protein k... | NM_178084.4 | 59.4% | 61.9% | (many diffs) |
| 8 | mouse | 65964 | Map3k20 | mitogen-activated protein k... | NM_023057.5 | 47.4% | 43.6% | (many diffs) |
| 9 | mouse | 65964 | Map3k20 | mitogen-activated protein k... | XM_006499997.2 | 47.4% | 43.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1431
- ORF length:
- 1365
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gtctctcggt gcctcctttg tgcaaattaa atttgatgac ttgcagtttt 121 ttgaaaactg cggtggagga agttttggga gtgtttatcg agccaaatgg atatcacagg 181 acaaggaggt ggctgtaaag aagctcctca aaatagagaa agaggcagaa atactcagtg 241 tcctcagtca cagaaacatc atccagtttt atggagtaat tcttgaacct cccaactatg 301 gcattgtcac agaatatgct tctctgggat cactctatga ttacattaac agtaacagaa 361 gtgaggagat ggatatggat cacattatga cctgggccac tgatgtagcc aaaggaatgc 421 attatttaca tatggaggct cctgtcaagg tgattcacag agacctcaag tcaagaaacg 481 ttgttatagc tgctgatgga gtattgaaga tctgtgactt tggtgcctct cggttccata 541 accatacaac acacatgtcc ttggttggaa ctttcccatg gatggctcca gaagttatcc 601 agagtctccc tgtgtcagaa acttgtgaca catattccta tggtgtggtt ctctgggaga 661 tgctaacaag ggaggtcccc tttaaaggtt tggaaggatt acaagtagct tggcttgtag 721 tggaaaaaaa cgagagatta accattccaa gcagttgccc cagaagtttt gctgaactgt 781 tacatcagtg ttgggaagct gatgccaaga aacggccatc attcaagcaa atcatttcaa 841 tcctggagtc catgtcaaat gacacgagcc ttcctgacaa gtgtaactca ttcctacaca 901 acaaggcgga gtggaggtgc gaaattgagg caactcttGA GAGGCTAAAG AAACTAGAGC 961 GTGATCTCAG CTTTAAGGAG CAGGAGCTTA AAGAACGAGA AAGACGTTTA AAGATGTGGG 1021 AGCAAAAGCT GACAGAGCAG TCCAACACCC CGCTTCTCTT GCCTCTTGCT GCAAGAATGT 1081 CTGAGGAGTC TTACTTTGAA TCTAAAACAG AGGAGTCAAA CAGTGCAGAG ATGTCATGTC 1141 AGATCACAGC AACAAGTAAC GGGGAGGGCC ATGGCATGAA CCCAAGTCTG CAGGCCATGA 1201 TGCTGATGGG CTTTGGGGAT ATCTTCTCAA TGAACAAAGC AGGAGCTGTG ATGCATTCTG 1261 GGATGCAGAT AAACATGCAA GCCAAGCAGA ATTCTTCCAA AACCACATCT AAGAGAAGGG 1321 GGAAGAAAGT CAACATGGCT CTGGGGTTCA GTGATTTTGA CTTGTCAGAA GGTGACGATG 1381 ATGATGATGA TGACGGTGAG GAGGAGGATA ATGACATGGA TAATAGTGAA TGCCCAACTT 1441 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1501 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1561 CTTGTGGAAA GGACGACTCA GGTTCCCCGA GCCGGTACAA ACGCGTTAAG TCgacaatca 1621 acctctggat tacaaaattt gtgaaagatt