Construct: ORF TRCN0000473778
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018004.1_s317c1
- Derived from:
- ccsbBroadEn_15482
- DNA Barcode:
- AGGTCCAACCAACTACATCTCCAT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF3E (3646)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473778
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3646 | EIF3E | eukaryotic translation init... | NM_001568.3 | 99.7% | 99.1% | 1101T>C;1206G>A;1323delT |
| 2 | human | 3646 | EIF3E | eukaryotic translation init... | XM_017013398.2 | 96.9% | 94.3% | (many diffs) |
| 3 | mouse | 16341 | Eif3e | eukaryotic translation init... | NM_008388.2 | 90.6% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1401
- ORF length:
- 1335
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagtacgac ttgactactc gcatcgcgca ctttttggat cggcatctag 121 tctttccgct tcttgaattt ctctctgtaa aggagatata taatgaaaag gaattattac 181 aaggtaaatt ggaccttctt agtgatacca acatggtaga ctttgctatg gatgtataca 241 aaaaccttta ttctgatgat attcctcatg ctttgagaga gaaaagaacc acagtggttg 301 cacaactgaa acagcttcag gcagaaacag aaccaattgt gaagatgttt gaagatccag 361 aaactacaag gcaaatgcag tcaaccaggg atggtaggat gctctttgac tacctggcgg 421 acaagcatgg ttttaggcag gaatatttag atacactcta cagatatgca aaattccagt 481 acgaatgtgg gaattactca ggagcagcag aatatcttta tttttttaga gtgctggttc 541 cagcaacaga tagaaatgct ttaagttcac tctggggaaa gctggcctct gaaatcttaa 601 tgcagaattg ggatgcagcc atggaagacc ttacacggtt aaaagagacc atagataata 661 attctgtgag ttctccactt cagtctcttc agcagagaac atggctcatt cactggtctc 721 tgtttgtttt cttcaatcac cccaaaggtc gcgataatat tattgacctc ttcctttatc 781 agccacaata tcttaatgca attcagacaa tgtGTCCACA CATTCTTCGC TATTTGACTA 841 CAGCAGTCAT AACAAACAAG GATGTTCGAA AACGTCGGCA GGTTCTAAAA GATCTAGTTA 901 AAGTTATTCA ACAGGAGTCT TACACATATA AAGACCCAAT TACAGAATTT GTTGAATGTT 961 TATATGTTAA CTTTGACTTT GATGGGGCTC AGAAAAAGCT GAGGGAATGT GAATCAGTGC 1021 TTGTGAATGA CTTCTTCTTG GTGGCTTGTC TTGAGGATTT CATTGAAAAT GCCCGTCTCT 1081 TCATATTTGA GACTTTCTGT CGCATCCACC AGTGTATCAG CATTAACATG TTGGCAGATA 1141 AATTGAACAT GACTCCAGAA GAAGCCGAAA GGTGGATTGT AAATTTGATT AGAAATGCAA 1201 GACTGGATGC CAAGATTGAT TCTAAATTAG GTCATGTGGT TATGGGTAAC AATGCAGTCT 1261 CACCCTATCA ACAAGTGATT GAAAAGACCA AAAGCCTTTC CTTTAGAAGC CAGATGTTGG 1321 CCATGAATAT TGAGAAGAAA CTTAATCAGA ATAGCAGGTC AGAGGCTCCT AACTGGGCAA 1381 CTCAAGATCT GGCTTCTACT GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 1441 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 1501 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGGTC CAACCAACTA 1561 CATCTCCATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt