Construct: ORF TRCN0000473780
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000887.1_s317c1
- Derived from:
- ccsbBroadEn_09162
- DNA Barcode:
- CGGCTGGCTCGACCGTGGAGCATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FYTTD1 (84248)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473780
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84248 | FYTTD1 | forty-two-three domain cont... | NM_032288.7 | 99.8% | 99.6% | 260G>A |
| 2 | human | 84248 | FYTTD1 | forty-two-three domain cont... | NM_001011537.2 | 90.2% | 88.1% | (many diffs) |
| 3 | human | 84248 | FYTTD1 | forty-two-three domain cont... | NR_027840.2 | 13% | (many diffs) | |
| 4 | mouse | 69823 | Fyttd1 | forty-two-three domain cont... | NM_027226.4 | 87.8% | 92.1% | (many diffs) |
| 5 | mouse | 69823 | Fyttd1 | forty-two-three domain cont... | XM_006522521.3 | 80.8% | 84.2% | (many diffs) |
| 6 | mouse | 69823 | Fyttd1 | forty-two-three domain cont... | NM_001159349.1 | 77.9% | 81.7% | (many diffs) |
| 7 | mouse | 69823 | Fyttd1 | forty-two-three domain cont... | XM_006522522.3 | 69.7% | 72.6% | (many diffs) |
| 8 | mouse | 69823 | Fyttd1 | forty-two-three domain cont... | XM_006522523.3 | 61.4% | 61.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccggtttggt acccggttgg tgggagccac ggcgacttct tcgccgccgc 121 cgaaggcccg cagcaatgaa aacctcgaca aaatagatat gtctttggat gatatcatca 181 agttgaatcg aaaggaaggg aagaagcaga attttccaag actaaataga agactcctcc 241 agcaaagtgg tgcccagcaa ttcaggatga gagtgcgatg gggaatccaa cagaattctg 301 gttttggtaa gactagtctg aatcatagag gaagagtaat gcctggaaag agacgtccta 361 atggagttat cactggcctt gcagctagga aaacgactgg aattcgaaaa ggaattagtc 421 ctatgaatcg tccacctcta agtgacaaga atatagaaca atattttcca gtgttaaaaa 481 ggaaggcaaa ccttcTGAGA CAAAATGAAG GGCAGAGGAA ACCAGTAGCA GTTCTCAAGA 541 GACCTAGCCA GCTAAGCAGA AAAAATAACA TTCCAGCTAA TTTTACCAGG AGTGGAAATA 601 AATTAAATCA TCAGAAAGAT ACTCGTCAGG CAACTTTTCT TTTCAGAAGA GGCCTGAAGG 661 TGCAGGCCCA GTTGAATACA GAACAACTGC TAGACGATGT AGTAGCAAAG AGAACTCGTC 721 AATGGCGGAC TTCCACCACA AATGGAGGGA TTTTGACTGT ATCTATTGAC AATCCTGGAG 781 CAGTGCAATG CCCAGTAACT CAGAAACCAC GATTAACTCG TACTGCTGTA CCTTCATTTT 841 TAACAAAGCG GGAGCAAAGT GACGTCAAGA AAGTTCCTAA AGGTGTTCCC CTGCAGTTTG 901 ACATAAACAG TGTCGGAAAA CAGACAGGGA TGACGTTGAA TGAGCGGTTT GGGATCCTGA 961 AGGAACAAAG AGCCACTCTC ACATACAACA AAGGGGGAAG CCGCTTTGTC ACCGTGGGAT 1021 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGACGGCT GGCTCGACCG TGGAGCATTA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt