Construct: ORF TRCN0000473805
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006645.1_s317c1
- Derived from:
- ccsbBroadEn_01543
- DNA Barcode:
- ACCCCTTGAGGAGTACAGCTTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC2A5 (6518)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473805
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6518 | SLC2A5 | solute carrier family 2 mem... | NM_001328619.2 | 100% | 100% | |
| 2 | human | 6518 | SLC2A5 | solute carrier family 2 mem... | NM_003039.3 | 100% | 100% | |
| 3 | human | 6518 | SLC2A5 | solute carrier family 2 mem... | NM_001328620.2 | 91.2% | 88.8% | 0_1ins94;37_38ins38 |
| 4 | human | 6518 | SLC2A5 | solute carrier family 2 mem... | NM_001328621.2 | 70.6% | 70.6% | 0_1ins441 |
| 5 | human | 6518 | SLC2A5 | solute carrier family 2 mem... | NM_001135585.2 | 45.4% | 38% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcaacaggat cagagcatga aggaagggag gctgacgctt gtgcttgccc 121 tggcaaccct gatagctgcc tttgggtcat ccttccagta tgggtacaac gtggctgctg 181 tcaactcccc agcactgctc atgcaacaat tttacaatga gacttactat ggtaggaccg 241 gtgaattcat ggaagacttc cccttgacgt tgctgtggtc tgtaaccgtg tccatgtttc 301 catttggagg gtttatcgga tccctcctgg tcggcccctt ggtgaataaa tttggcagaa 361 aaggggcctt gctgttcaac aacatatttt ctatcgtgcc tgcgatctta atgggatgca 421 gcagagtcgc cacatcattt gagcttatca ttatttccag acttttggtg ggaatatgtg 481 caggtgtatc ttccaacgtg gtccccatgt acttagggga gctggcccct aaaaacctgc 541 ggggggctct cggggtggtg ccccagctct tcatcactgt tggcatcctt gtggcccaga 601 tctttggtct tcggaatctc cttgcaaacg tagatggctg gccgatcctg ctggggctga 661 ccggggtccc cgcggcgctg cagctccttc tgctgccctt cttccccgag agccccaggt 721 acctgctgat tcagaagaaa gacgaagcgg ccgccaagaa agccctacag acgctgcgcg 781 gctgggactc tgtggacagg gaggtggccg agatccggca ggaggatgag gcagagaagg 841 ccgcgggctt catctccgtg ctgaagctgt tccggatgcg ctcgctgcgc tggcagctgc 901 tgtccatcat cgtcctcatg ggcggccagc agctgtcggg cgtcaacgct atctactact 961 acgcggacca gatctacctg agcgccggcg tgccggagga gcacgtgcag tacgtgacgg 1021 ccggcaccgg ggccgtgaac gtggtcatga ccttctgcgc cgtgttcgtg gtggagctcc 1081 tgggtcggag gctgctgctg ctgctgggct tctccatctg cctcatagcc tgctgcgtgc 1141 tcactgcagc tctggcactg caggacacag tgtcctggat gccatacatc agcatcgtct 1201 gtgtcatctc ctacgtcata ggacatgccc TCGGGCCCAG TCCCATACCC GCGCTGCTCA 1261 TCACTGAGAT CTTCCTGCAG TCCTCTCGGC CATCTGCCTT CATGGTGGGG GGCAGTGTGC 1321 ACTGGCTCTC CAACTTCACC GTGGGCTTGA TCTTCCCGTT CATCCAGGAG GGCCTCGGCC 1381 CGTACAGCTT CATTGTCTTC GCCGTGATCT GCCTCCTCAC CACCATCTAC ATCTTCTTGA 1441 TTGTCCCGGA GACCAAGGCC AAGACGTTCA TAGAGATCAA CCAGATTTTC ACCAAGATGA 1501 ATAAGGTGTC TGAAGTGTAC CCGGAAAAGG AGGAACTGAA AGAGCTTCCA CCTGTCACTT 1561 CGGAACAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACCCCT TGAGGAGTAC AGCTTATGAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt