Construct: ORF TRCN0000473810
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014481.1_s317c1
- Derived from:
- ccsbBroadEn_03734
- DNA Barcode:
- ACTGCTACCCACAGAAGCTTTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM39 (56658)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473810
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 56658 | TRIM39 | tripartite motif containing 39 | NM_001369521.1 | 100% | 100% | |
2 | human | 56658 | TRIM39 | tripartite motif containing 39 | NM_001369522.1 | 100% | 100% | |
3 | human | 56658 | TRIM39 | tripartite motif containing 39 | NM_001369523.1 | 100% | 100% | |
4 | human | 56658 | TRIM39 | tripartite motif containing 39 | NM_172016.2 | 100% | 100% | |
5 | human | 56658 | TRIM39 | tripartite motif containing 39 | NM_021253.4 | 94.2% | 94.2% | 804_893del |
6 | human | 202658 | TRIM39-RPP21 | TRIM39-RPP21 readthrough | NM_001199119.1 | 82.7% | 74.1% | (many diffs) |
7 | mouse | 79263 | Trim39 | tripartite motif-containing 39 | NM_024468.2 | 90.4% | 97.5% | (many diffs) |
8 | mouse | 79263 | Trim39 | tripartite motif-containing 39 | NM_178281.1 | 90.4% | 97.5% | (many diffs) |
9 | mouse | 79263 | Trim39 | tripartite motif-containing 39 | XM_006525171.3 | 88.9% | 95.9% | (many diffs) |
10 | mouse | 79263 | Trim39 | tripartite motif-containing 39 | XM_006525172.3 | 47.7% | 51% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1530
- ORF length:
- 1464
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagacaagt ctgttagagg ctggggcctc tgcagcctct acagctgcgg 121 ctttggagaa cttacaggtg gaggcgagct gctctgtgtg cctggagtat ctgaaggaac 181 ctgtcatcat tgagtgtggg cacaacttct gcaaagcttg catcacccgc tggtgggagg 241 acctagagag ggacttccct tgtcctgtct gtcgaaagac atcccgctac cgcagtctcc 301 gacctaatcg gcaactaggc agtatggtgg aaattgccaa gcagctccag gccgtcaagc 361 ggaagatccg ggatgagagc ctctgccccc aacaccatga ggccctcagc cttttctgtt 421 atgaggacca ggaggctgta tgcttgatat gtgcaatttc ccacacccac cgggcccaca 481 ccgttgtgcc actggacgat gctacacagg agtacaagga aaaactgcag aagtgtctgg 541 agcccctgga acagaagctg caggagatca ctcgctgcaa gtcctctgag gagaagaagc 601 ctggtgagct caagagacta gtggaaagtc gccgacagca gatcttgagg gagtttgaag 661 agcttcatag gcggctggat gaagagcagc aggtgttgct ttcacgactg gaagaagagg 721 aacaggacat tctgcagcga ctccgagaaa atgctgctca ccttggggac aagcgccggg 781 acctggccca cttggctgcc gaggtggagg gcaagtgctt acagtcaggc ttcgagatgc 841 ttaaggatgt caaaagtacc ctggaaaaat gtgaaaaggt gaagaccatg gaggtgactt 901 cagtatccat agagctggaa aagaacttca gcaattttcc ccgacagtac tttgccctaa 961 ggaaaatcct taaacagcta attgcggatg tgaccctgga ccctgagaca gctcatccta 1021 acctagtcct gtcagaggat cgtaagagcg tcaagttcgt ggagacaaga ctccgggatc 1081 tccctgacac accaaggcgt ttcaccttct acccttgcgt cctggctact gagggtttca 1141 cctcaggtcg aCACTACTGG GAGGTGGAGG TGGGCGACAA GACCCACTGG GCAGTGGGTG 1201 TATGCCGGGA CTCCGTGAGC CGAAAGGGCG AGTTGACTCC ACTCCCTGAG ACTGGCTACT 1261 GGCGGGTGCG GCTATGGAAT GGGGACAAAT ATGCAGCCAC CACCACACCT TTTACCCCTT 1321 TGCACATCAA GGTGAAACCC AAGCGGGTAG GCATATTCCT AGACTATGAG GCCGGCACAC 1381 TGTCTTTCTA CAATGTCACA GACCGCTCTC ATATCTACAC CTTCACTGAT ACTTTTACTG 1441 AGAAACTTTG GCCCCTCTTC TACCCAGGCA TCCGGGCTGG ACGGAAGAAT GCTGCACCAC 1501 TTACCATCAG GCCCCCAACA GATTGGGAGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1561 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1621 CGTAACTTGA AAGTATTTCG ATTTCTGGCT TTATATATCT TGTGGAAAGG ACGAACTGCT 1681 ACCCACAGAA GCTTTACCAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 1741 gaaagatt