Construct: ORF TRCN0000473815
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002241.1_s317c1
- Derived from:
- ccsbBroadEn_12200
- DNA Barcode:
- CGAACTAACCGGTGGTTCGGGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UEVLD (55293)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473815
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55293 | UEVLD | UEV and lactate/malate dehy... | NM_001297771.3 | 82.3% | 82.3% | 1_114del |
| 2 | human | 55293 | UEVLD | UEV and lactate/malate dehy... | NM_001261385.2 | 51.2% | 47.7% | (many diffs) |
| 3 | human | 55293 | UEVLD | UEV and lactate/malate dehy... | NM_018314.6 | 46% | 43% | (many diffs) |
| 4 | human | 55293 | UEVLD | UEV and lactate/malate dehy... | NM_001261383.3 | 44.9% | 40.4% | (many diffs) |
| 5 | human | 55293 | UEVLD | UEV and lactate/malate dehy... | NM_001040697.4 | 37% | 34.7% | (many diffs) |
| 6 | human | 55293 | UEVLD | UEV and lactate/malate dehy... | NM_001261382.3 | 35.8% | 32.3% | (many diffs) |
| 7 | human | 55293 | UEVLD | UEV and lactate/malate dehy... | NM_001261386.3 | 24.9% | 21.7% | (many diffs) |
| 8 | human | 55293 | UEVLD | UEV and lactate/malate dehy... | NM_001261384.3 | 19% | 16.9% | (many diffs) |
| 9 | mouse | 54122 | Uevld | UEV and lactate/malate dehy... | XM_006540993.3 | 40.5% | 37.9% | (many diffs) |
| 10 | mouse | 54122 | Uevld | UEV and lactate/malate dehy... | XM_006540994.3 | 40.5% | 37.9% | (many diffs) |
| 11 | mouse | 54122 | Uevld | UEV and lactate/malate dehy... | XR_391351.3 | 33.3% | (many diffs) | |
| 12 | mouse | 54122 | Uevld | UEV and lactate/malate dehy... | NM_001040695.1 | 32.3% | 30.4% | (many diffs) |
| 13 | mouse | 54122 | Uevld | UEV and lactate/malate dehy... | XR_391352.3 | 32.2% | (many diffs) | |
| 14 | mouse | 54122 | Uevld | UEV and lactate/malate dehy... | XR_001785588.1 | 10.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 597
- ORF length:
- 531
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cacctatgtt tttaaagata gttctcagaa agacctgctg aattttactg 121 gcacaattcc tgtgatgtat cagggtaata catataacat accaattcgt ttctggattt 181 tGGATTCTCA CCCTTTCGCT CCCCCTATTT GCTTCTTGAA GCCAACTGCA AATATGGGAA 241 TCTTAGTCGG AAAACATGTG GATGCTCAAG GCAGAATATA TTTGCCCTAT CTCCAAAACT 301 GGAGCCATCC TAAATCTGTC ATTGTTGGAT TAATTAAAGA AATGATTGCC AAGTTTCAAG 361 AGGAACTTCC CATGTATTCT CTATCATCAT CTGATGAGGC ACGGCAGGTA GACTTGCTAG 421 CCTATATTGC AAAAATCACT GAAGGTGTTT CAGATACAAA TTCAAAGAGC TGGGCAAATC 481 ATGAGAATAA AACAGTCAAT AAAATTACTG TGGTTGGAGG TGGAGAACTC GGTATTGCCT 541 GCACATTAGC AATTTCAGCA AAGGTGGAGT CTCGCTCTGT TACCCAGGCT GGAGTGTACC 601 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 661 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 721 ATATATCTTG TGGAAAGGAC GACGAACTAA CCGGTGGTTC GGGGGAACGC GTTAAGTCga 781 caatcaacct ctggattaca aaatttgtga aagatt