Construct: ORF TRCN0000473815

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002241.1_s317c1
Derived from:
ccsbBroadEn_12200
DNA Barcode:
CGAACTAACCGGTGGTTCGGGGGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UEVLD (55293)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473815
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55293 UEVLD UEV and lactate/malate dehy... NM_001297771.3 82.3% 82.3% 1_114del
2 human 55293 UEVLD UEV and lactate/malate dehy... NM_001261385.2 51.2% 47.7% (many diffs)
3 human 55293 UEVLD UEV and lactate/malate dehy... NM_018314.6 46% 43% (many diffs)
4 human 55293 UEVLD UEV and lactate/malate dehy... NM_001261383.3 44.9% 40.4% (many diffs)
5 human 55293 UEVLD UEV and lactate/malate dehy... NM_001040697.4 37% 34.7% (many diffs)
6 human 55293 UEVLD UEV and lactate/malate dehy... NM_001261382.3 35.8% 32.3% (many diffs)
7 human 55293 UEVLD UEV and lactate/malate dehy... NM_001261386.3 24.9% 21.7% (many diffs)
8 human 55293 UEVLD UEV and lactate/malate dehy... NM_001261384.3 19% 16.9% (many diffs)
9 mouse 54122 Uevld UEV and lactate/malate dehy... XM_006540993.3 40.5% 37.9% (many diffs)
10 mouse 54122 Uevld UEV and lactate/malate dehy... XM_006540994.3 40.5% 37.9% (many diffs)
11 mouse 54122 Uevld UEV and lactate/malate dehy... XR_391351.3 33.3% (many diffs)
12 mouse 54122 Uevld UEV and lactate/malate dehy... NM_001040695.1 32.3% 30.4% (many diffs)
13 mouse 54122 Uevld UEV and lactate/malate dehy... XR_391352.3 32.2% (many diffs)
14 mouse 54122 Uevld UEV and lactate/malate dehy... XR_001785588.1 10.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
597
ORF length:
531
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga cacctatgtt tttaaagata gttctcagaa agacctgctg aattttactg
121gcacaattcc tgtgatgtat cagggtaata catataacat accaattcgt ttctggattt
181tGGATTCTCA CCCTTTCGCT CCCCCTATTT GCTTCTTGAA GCCAACTGCA AATATGGGAA
241TCTTAGTCGG AAAACATGTG GATGCTCAAG GCAGAATATA TTTGCCCTAT CTCCAAAACT
301GGAGCCATCC TAAATCTGTC ATTGTTGGAT TAATTAAAGA AATGATTGCC AAGTTTCAAG
361AGGAACTTCC CATGTATTCT CTATCATCAT CTGATGAGGC ACGGCAGGTA GACTTGCTAG
421CCTATATTGC AAAAATCACT GAAGGTGTTT CAGATACAAA TTCAAAGAGC TGGGCAAATC
481ATGAGAATAA AACAGTCAAT AAAATTACTG TGGTTGGAGG TGGAGAACTC GGTATTGCCT
541GCACATTAGC AATTTCAGCA AAGGTGGAGT CTCGCTCTGT TACCCAGGCT GGAGTGTACC
601CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
661TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
721ATATATCTTG TGGAAAGGAC GACGAACTAA CCGGTGGTTC GGGGGAACGC GTTAAGTCga
781caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)