Construct: ORF TRCN0000473821
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018555.1_s317c1
- Derived from:
- ccsbBroadEn_06917
- DNA Barcode:
- GCACTCCTTATTAGGAGCTCTTAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SCTR (6344)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473821
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6344 | SCTR | secretin receptor | NM_002980.2 | 99.7% | 60% | 652G>A;826_827insC;1176T>C |
| 2 | human | 6344 | SCTR | secretin receptor | XM_011511621.2 | 98.6% | 59.8% | (many diffs) |
| 3 | human | 6344 | SCTR | secretin receptor | XM_017004670.1 | 94.7% | 55.1% | (many diffs) |
| 4 | human | 6344 | SCTR | secretin receptor | XM_017004671.1 | 90.5% | 52.1% | (many diffs) |
| 5 | human | 6344 | SCTR | secretin receptor | XM_024453038.1 | 84.3% | 45.3% | (many diffs) |
| 6 | human | 6344 | SCTR | secretin receptor | XM_017004672.1 | 83.3% | 45.2% | (many diffs) |
| 7 | human | 6344 | SCTR | secretin receptor | XR_922984.2 | 66.7% | (many diffs) | |
| 8 | human | 6344 | SCTR | secretin receptor | XR_001738887.1 | 62.6% | (many diffs) | |
| 9 | human | 6344 | SCTR | secretin receptor | XR_001738889.1 | 60.2% | (many diffs) | |
| 10 | human | 6344 | SCTR | secretin receptor | XM_005263730.5 | 59.3% | 21.5% | (many diffs) |
| 11 | human | 6344 | SCTR | secretin receptor | XM_017004673.1 | 58.6% | 21.7% | (many diffs) |
| 12 | human | 6344 | SCTR | secretin receptor | XR_001738888.1 | 54% | (many diffs) | |
| 13 | mouse | 319229 | Sctr | secretin receptor | XM_006529688.3 | 70.3% | 37.2% | (many diffs) |
| 14 | mouse | 319229 | Sctr | secretin receptor | XM_011248029.1 | 70.3% | 37.2% | (many diffs) |
| 15 | mouse | 319229 | Sctr | secretin receptor | XM_011248030.2 | 70.3% | 37.2% | (many diffs) |
| 16 | mouse | 319229 | Sctr | secretin receptor | XM_011248031.2 | 66.6% | 34.1% | (many diffs) |
| 17 | mouse | 319229 | Sctr | secretin receptor | XM_006529689.3 | 58.5% | 23.7% | (many diffs) |
| 18 | mouse | 319229 | Sctr | secretin receptor | XM_006529690.3 | 42.9% | 11.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 990
- ORF length:
- 924
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg tccccacctg tcgccgccgc tgcagcagct actactgccg gtgctgctcg 121 cctgcgccgc gcactcgact ggagcccttc cccgactatg tgacgtgcta caagtgctgt 181 gggaagagca agaccagtgc ctgcaggaac tctccagaga gcagacagga gacctgggca 241 cggagcagcc agtgccaggt tgtgagggga tgtgggacaa cataagctgc tggccctctt 301 ctgtgccggg ccggatggtg gaggtggaat gcccgagatt cctccggatg ctcaccagca 361 gaaatggttc cttgttccga aactgcacac aggatggctg gtcagaaacc ttccccaggc 421 ctaatctggc ctgtggcgtt aatgtgaacg actcttccaa cgagaagcgg cactcctacc 481 tgctgaagct gaaagtcatg tacaccgtgg gctacagctc ctccctggtc atgctcctgg 541 tcgcccttgg catcctctgt gctttccgga ggctccactg cactcgcaac tacatccaca 601 tgcacctgtt cgtgtccttc atccttcgtg ccctgtccaa cttcatcaag gacgccgtgc 661 tcttctcctc agatgatgtc acctactgcg atgcccacag ggcgggctgc aagctgatca 721 tggtgctgtt ccagtactgc atcatggcca actactcctg gctgctggtg gaaggcctct 781 accttcacac actcctcgcc atctccttct tctctgaaag aaagtacctc cagggatttg 841 tggcattcgg atggggttct ccagCCATTT TTGTTGCTTT GTGGGCTATT GCCCAGACAC 901 TTTCTGGAAG ATGTTGGGTG CTGGGACATC AATGCCAACG CATCCATCTG GTGGATCATT 961 CGTGGTCCTG TGATCCTCTC CATCCTGATT AATTTCATCC TTTTCATAAA CATTCTAAGA 1021 ATCCTGATGA GAAAACTTAG AACCCAAGAA ACAAGAGGAA ATGAAGTCAG CCATTATAAG 1081 CGCCTGGCCA GGTCCACTCT CCTGCTGATC CCCCTCTTTG GCATCCACTA CATCGTCTTC 1141 GCCTTCTCCC CAGAGGACGC TATGGAGATC CAGCTGTTTT TTGAACTAGC CCTTGGCTCA 1201 TTCCAGGGAC TGGTGGTGGC CGTCCTCTAC TGCTTCCTCA ACGGGGAGGT GCAGCTGGAG 1261 GTTCAGAAGA AGTGGCAGCA ATGGCACCTC CGTGAGTTCC CACTGCACCC CGTGGCCTCC 1321 TTCAGCAACA GCACCAAGGC CAGCCACTTG GAGCAGAGCC AGGGCACCTG CAGGACCAGC 1381 ATCATCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT 1441 CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT 1501 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCACTCCT TATTAGGAGC TCTTAAACGC 1561 GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt