Construct: ORF TRCN0000473822
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006755.1_s317c1
- Derived from:
- ccsbBroadEn_00471
- DNA Barcode:
- GAGAAGAATGCTGTACAACCTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- E2F6 (1876)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473822
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1876 | E2F6 | E2F transcription factor 6 | NM_198256.4 | 100% | 100% | |
| 2 | human | 1876 | E2F6 | E2F transcription factor 6 | NM_001278275.1 | 88.2% | 87.5% | (many diffs) |
| 3 | human | 1876 | E2F6 | E2F transcription factor 6 | XM_017003547.1 | 78.3% | 65.4% | (many diffs) |
| 4 | human | 1876 | E2F6 | E2F transcription factor 6 | NM_001278276.2 | 73.3% | 73.3% | 0_1ins225 |
| 5 | human | 1876 | E2F6 | E2F transcription factor 6 | NM_001278277.1 | 73.3% | 73.3% | 0_1ins225 |
| 6 | human | 1876 | E2F6 | E2F transcription factor 6 | NM_001278278.1 | 73.3% | 73.3% | 0_1ins225 |
| 7 | human | 1876 | E2F6 | E2F transcription factor 6 | XM_017003548.1 | 68.3% | 55.2% | (many diffs) |
| 8 | human | 1876 | E2F6 | E2F transcription factor 6 | XM_017003549.2 | 55.6% | 43.5% | (many diffs) |
| 9 | human | 1876 | E2F6 | E2F transcription factor 6 | NM_212540.3 | 45.9% | 45.9% | 0_1ins456 |
| 10 | human | 102724563 | LOC102724563 | transcription factor E2F6 p... | XR_002958854.1 | 36.3% | (many diffs) | |
| 11 | human | 102724865 | LOC102724865 | transcription factor E2F6 p... | XR_001756157.2 | 28.2% | (many diffs) | |
| 12 | human | 1876 | E2F6 | E2F transcription factor 6 | XR_001738660.1 | 26.2% | 1_282del;934_937delGTAG;1130_3214del | |
| 13 | human | 1876 | E2F6 | E2F transcription factor 6 | NR_103490.2 | 17.7% | 1_282del;662_663ins271;855_2959del | |
| 14 | mouse | 50496 | E2f6 | E2F transcription factor 6 | NM_033270.2 | 79.5% | 84.3% | (many diffs) |
| 15 | mouse | 50496 | E2f6 | E2F transcription factor 6 | XM_006515147.1 | 69.6% | 74% | (many diffs) |
| 16 | mouse | 50496 | E2f6 | E2F transcription factor 6 | XM_006515148.1 | 69.6% | 74% | (many diffs) |
| 17 | mouse | 50496 | E2f6 | E2F transcription factor 6 | XM_006515149.3 | 64.1% | 68.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 909
- ORF length:
- 843
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tcagcagcgg ccggcgagga agttacccag tctcctcctg gacccgacgg 121 aggagacggt tcgccgtcgg tgccgagacc ccatcaacgt ggagggcctg ctgccatcaa 181 aaataaggat taatttagaa gataatgtac aatatgtgtc catgagaaaa gctctaaaag 241 tgaagagacc tcgttttgat gtatcgctgg tttatttaac tcgaaaattt atggatcttg 301 tcagatctgc tcccgggggt attcttgact taaacaaggt tgcaacgaaa ctgggagtcc 361 gaaagcggag agtgtatgac atcaccaatg tcttagatgg aatcgacctc gttgaaaaga 421 aaTCCAAGAA CCATATTAGA TGGATAGGAT CTGATCTTAG CAATTTTGGA GCAGTTCCCC 481 AACAAAAGAA GCTACAGGAG GAACTTTCTG ACTTATCAGC AATGGAAGAT GCTTTGGATG 541 AGTTAATTAA GGATTGTGCT CAGCAGCTGT TTGAGTTAAC AGATGACAAA GAAAATGAAA 601 GACTAGCATA TGTGACCTAT CAAGACATTC ATAGCATTCA GGCCTTCCAT GAACAGATCG 661 TCATTGCAGT TAAAGCTCCA GCAGAAACCA GATTGGATGT TCCAGCTCCC AGAGAAGACT 721 CTATCACAGT GCACATAAGG AGCACCAACG GACCTATCGA TGTCTATTTG TGTGAAGTGG 781 AGCAGGGTCA GACCAGTAAC AAAAGGTCTG AAGGTGTCGG GACCTCTTCA TCTGAGAGCA 841 CTCATCCAGA AGGCCCTGAG GAAGAAGAAA ATCCTCAGCA AAGTGAAGAA TTGCTTGAAG 901 TAAGCAACTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 961 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1021 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGAGAAG AATGCTGTAC AACCTTGAAC 1081 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt