Construct: ORF TRCN0000473830
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007670.1_s317c1
- Derived from:
- ccsbBroadEn_10782
- DNA Barcode:
- ACATCAGTACCCCCGCAGATCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DMWD (1762)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473830
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1762 | DMWD | DM1 locus, WD repeat contai... | NM_004943.2 | 49.5% | 49.5% | 1_945del;1362C>G;1902_1976del |
| 2 | mouse | 13401 | Dmwd | dystrophia myotonica-contai... | XM_006539525.3 | 44.9% | 48% | (many diffs) |
| 3 | mouse | 13401 | Dmwd | dystrophia myotonica-contai... | XM_006539524.3 | 43.2% | 46.2% | (many diffs) |
| 4 | mouse | 13401 | Dmwd | dystrophia myotonica-contai... | NM_010058.2 | 41.6% | 44.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1068
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cctgcgtggg ctcatgaaga gctactttgg gggcctgctg tgtgtgtgct 121 ggagccctga cggccgctac gtggtgacgg gtggcgaaga tgacctggtc accgtgtggt 181 ccttcaccga gggccgcgtg gtggctcgag gccatggcca caagtcctgg gtcaacgctg 241 tggcctttga cccctacacc acaagggcag aggaggcggc gacagcagcc ggtgctgatg 301 gggagcggag cggcgaagag gaggaggagg agcccgaggc tgcgggcaca ggctcggccg 361 ggggcgcccc gctctctcca ctgcccaagg ctggctccat tacttaccgc tttggctcgg 421 cgggccagga cacgcagttc tgcctgtggg acctcactga agacgtgctc tacccgcacc 481 cgcccctggc ccgcacccgc accctccctg gcacacctgg caccacgcca ccggccgcca 541 gcagctcgag gggtggcgag cctggcccag gccccctgcc tcgctcgctg tcccgctcca 601 acagtctccc gcacccagct ggcgggggca aggcgggCGG CCCGGGTGTG GCGGCAGAGC 661 CTGGCACACC ATTCAGCATT GGCCGCTTCG CCACGCTCAC ACTGCAGGAG CGGCGGGACC 721 GGGGGGCAGA GAAGGAGCAC AAGCGCTACC ACAGCCTGGG CAACATCAGC CGGGGTGGCA 781 GTGGCGGCAG TGGCAGTGGT GGGGAGAAGC CCAGCGGCCC TGTTCCCCGC AGCCGCCTGG 841 ACCCCGCCAA GGTGCTGGGC ACTGCGCTGT GCCCGCGCAT CCACGAGGTG CCCCTGCTGG 901 AGCCCCTTGT GTGCAAGAAG ATCGCCCAGG AGCGGCTCAC AGTCCTCCTG TTCCTGGAGG 961 ACTGCATCAT CACTGCCTGC CAGGAGGGCC TCATCTGCAC CTGGGCCCGG CCGGGCAAGG 1021 CGGGCATCTC CTCCCAACCA GGCAACTCCC CGAGTGGCAC AGTGGTGTGC CCAACTTTCT 1081 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1141 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1201 GTGGAAAGGA CGAACATCAG TACCCCCGCA GATCGTCACG CGTTAAGTCg acaatcaacc 1261 tctggattac aaaatttgtg aaagatt