Construct: ORF TRCN0000473834
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014137.1_s317c1
- Derived from:
- ccsbBroadEn_12062
- DNA Barcode:
- GCAACGTATCGCATTATTCTCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGLN1 (54583)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473834
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54583 | EGLN1 | egl-9 family hypoxia induci... | NM_022051.2 | 30% | 30% | 1_894del |
| 2 | human | 54583 | EGLN1 | egl-9 family hypoxia induci... | XM_005273166.5 | 19.9% | 19.9% | 1_894del;1149_1150ins129 |
| 3 | mouse | 112405 | Egln1 | egl-9 family hypoxia-induci... | NM_053207.2 | 28.9% | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgaTC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGT TGCTTGTTAT CCGGGCAATG GAACGGGTTA TGTACGTCAT GTTGATAATC 121 CAAATGGAGA TGGAAGATGT GTGACATGTA TATATTATCT TAATAAAGAC TGGGATGCCA 181 AGGTAAGTGG AGGTATACTT CGAATTTTTC CAGAAGGCAA AGCCCAGTTT GCTGACATTG 241 AACCCAAATT TGATAGACTG CTGTTTTTCT GGTCTGACCG TCGCAACCCT CATGAAGTAC 301 AACCAGCATA TGCTACAAGG TACGCAATAA CTGTTTGGTA TTTTGATGCA GATGAGAGAG 361 CACGAGCTAA AGTAAAATAT CTAACAGGTG AAAAAGGTGT GAGGGTTGAA CTCAATAAAC 421 CTTCAGATTC GGTCGGTAAA GACGTCTTCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCAAC 601 GTATCGCATT ATTCTCGACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt