Construct: ORF TRCN0000473836
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010831.1_s317c1
- Derived from:
- ccsbBroadEn_15754
- DNA Barcode:
- ATTGTGCGAGGCATCCGAGAACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASTN2 (23245)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473836
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23245 | ASTN2 | astrotactin 2 | NM_198188.2 | 100% | 100% | |
| 2 | human | 23245 | ASTN2 | astrotactin 2 | NM_198187.3 | 98.2% | 98.2% | 1152_1172del |
| 3 | human | 23245 | ASTN2 | astrotactin 2 | NM_001184734.1 | 94.9% | 94.4% | 1117_1118ins34;1125_1126ins26 |
| 4 | human | 23245 | ASTN2 | astrotactin 2 | NM_198186.3 | 86.9% | 86.7% | (many diffs) |
| 5 | human | 23245 | ASTN2 | astrotactin 2 | NM_001184735.1 | 47.1% | 44.1% | (many diffs) |
| 6 | human | 23245 | ASTN2 | astrotactin 2 | NM_014010.5 | 29.9% | 29.7% | (many diffs) |
| 7 | human | 23245 | ASTN2 | astrotactin 2 | NM_001365069.1 | 28.8% | 28.7% | (many diffs) |
| 8 | human | 23245 | ASTN2 | astrotactin 2 | NM_001365068.1 | 28.8% | 28.6% | (many diffs) |
| 9 | mouse | 56079 | Astn2 | astrotactin 2 | NM_019514.3 | 26.9% | 29.1% | (many diffs) |
| 10 | mouse | 56079 | Astn2 | astrotactin 2 | XM_006538104.3 | 25.9% | 28.1% | (many diffs) |
| 11 | mouse | 56079 | Astn2 | astrotactin 2 | NM_207109.2 | 25.8% | 28% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1254
- ORF length:
- 1185
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccttcatc acctacctct caggtttgct gacagcccag atgctgtcag 121 atgaccagct catttcaggt gtggagattc gctgtgagga gaaggggcgc tgtccatcta 181 cctgtcacct ttgccgccgg ccaggcaagg agcagctgag ccccacacca gtgctgctgg 241 aaatcaaccg tgtggtgcca ctttataccc tcatccaaga caatggcaca aaggaggcct 301 tcaagagtgc actgatgagt tcctactggt gctcagggaa aggggatgtg atcgatgact 361 ggtgcaggtg tgacctcagc gcctttgatg ccaatgggct ccccaactgc agcccccttc 421 tgcagccggt gctgcggctg tccccaacag tggagccctc cagtactgtg gtctccttgg 481 agtgggtgga tgttcagcca gctattggga ccaaggtctc cgactatatt ctgcagcata 541 agaaagtgga tgaatacaca gacactgacc tgtacacagg agaattcctg agttttgctg 601 atgacttact ctctggcctg ggcacatctt gtgtagcagc tggtcgaagc catggagagg 661 tccctgaagt cagtatctac tcagtcatct tcaagtgtct ggagcccgac ggtctctaca 721 agttcactct gtatgctgtg gatacacgag ggaggcactc agagctaagc acggtgaccc 781 tgaggacggc ctgtccactg gtagatgaca aCAAGGCAGA AGAAATAGCT GACAAGATCT 841 ACAATCTGTA CAATGGGTAC ACAAGTGGAA AGGAGCAGCA GATGGCCTAC AACACACTGA 901 TGGAGGTCTC AGCCTCGATG CTGTTCCGAG TCCAGCACCA CTACAACTCT CACTATGAAA 961 AGTTTGGCGA CTTCGTCTGG AGAAGTGAGG ATGAGCTGGG GCCCAGGAAG GCCCACCTGA 1021 TTCTACGGCG ACTGGAGAGG GTGAGTAGCC ACTGCTCCAG CCTCCTGCGG AGTGCCTACA 1081 TCCAGAGCCG CGTGGAAACA GTGCCCTATC TTTTCTGCCG CAGCGAGGAG GTCCGGCCTG 1141 CAGGCATGGT GTGGTATAGC ATCCTCAAGG ACACCAAAAT CACGTGTGAG GAGAAGATGG 1201 TGTCAATGGC CCGAAACACA TATTATTTGA CTCTATCAAA AGTCTCTCCT TTTTTGCCAA 1261 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1321 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1381 TATTTGTGGA AAGGACGAAT TGTGCGAGGC ATCCGAGAAC GTACGCGTTA AGTCgacaat 1441 caacctctgg attacaaaat ttgtgaaaga tt