Construct: ORF TRCN0000473840
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000529.1_s317c1
- Derived from:
- ccsbBroadEn_05822
- DNA Barcode:
- GCGGGAGGACGTCTCCATTAACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANXA1 (301)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473840
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 301 | ANXA1 | annexin A1 | NM_000700.3 | 99.9% | 100% | 327A>G |
| 2 | human | 301 | ANXA1 | annexin A1 | XM_011518609.1 | 99.9% | 100% | 327A>G |
| 3 | human | 301 | ANXA1 | annexin A1 | XM_017014657.1 | 96.8% | 96.9% | 1_33del;360A>G |
| 4 | mouse | 16952 | Anxa1 | annexin A1 | NM_010730.2 | 86.8% | 87.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1104
- ORF length:
- 1038
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aatggtatca gaattcctca agcaggcctg gtttattgaa aatgaagagc 121 aggaatatgt tcaaactgtg aagtcatcca aaggtggtcc cggatcagcg gtgagcccct 181 atcctacctt caatccatcc tcggatgtcg ctgccttgca taaggccata atggttaaag 241 gtgtggatga agcaaccatc attgacattc taactaagcg aaacaatgca cagcgtcaac 301 agatcaaagc agcatatctc caggaaacag gaaagcccct ggatgaaaca ctgaagaaag 361 cccttacagg tcaccttgag gaggttgttt tggctctgct aaaaactcca gcgcaatttg 421 atgctgatga acttcgtgct gccatgaagg gccttggaac tgatgaagat actctaattg 481 agattttggc atcaagaact aacaaagaaa tcagagacat taacagggtc tacagagagg 541 aactgaagag agatctggcc aaagacataa cctcagacac atctggagat tttcggaacg 601 ctttgctttc tcttgctaag ggtgaccgat ctgaggactt tggtgtgaat gaagacttgg 661 ctgattcaga tgccagggcc TTGTATGAAG CAGGAGAAAG GAGAAAGGGG ACAGACGTAA 721 ACGTGTTCAA TACCATCCTT ACCACCAGAA GCTATCCACA ACTTCGCAGA GTGTTTCAGA 781 AATACACCAA GTACAGTAAG CATGACATGA ACAAAGTTCT GGACCTGGAG TTGAAAGGTG 841 ACATTGAGAA ATGCCTCACA GCTATCGTGA AGTGCGCCAC AAGCAAACCA GCTTTCTTTG 901 CAGAGAAGCT TCATCAAGCC ATGAAAGGTG TTGGAACTCG CCATAAGGCA TTGATCAGGA 961 TTATGGTTTC CCGTTCTGAA ATTGACATGA ATGATATCAA AGCATTCTAT CAGAAGATGT 1021 ATGGTATCTC CCTTTGCCAA GCCATCCTGG ATGAAACCAA AGGAGATTAT GAGAAAATCC 1081 TGGTGGCTCT TTGTGGAGGA AACTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CGGGAGGACG 1261 TCTCCATTAA CTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att