Construct: ORF TRCN0000473845
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004847.1_s317c1
- Derived from:
- ccsbBroadEn_15957
- DNA Barcode:
- GCCGGTTTATTAATATTCTCTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM3A (60343)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473845
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_001171134.2 | 27.2% | 23.5% | (many diffs) |
| 2 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_005277879.4 | 27.2% | 23.5% | (many diffs) |
| 3 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_001171132.3 | 25.3% | 21.7% | (many diffs) |
| 4 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_001282312.2 | 25.3% | 21.7% | (many diffs) |
| 5 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_021806.4 | 25.3% | 21.7% | (many diffs) |
| 6 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_001363822.2 | 24.5% | 21.1% | (many diffs) |
| 7 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_006724832.3 | 24.4% | 21% | (many diffs) |
| 8 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_006724833.2 | 20.8% | 7.3% | 0_1ins56;155_681del |
| 9 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_024452418.1 | 20.8% | 7.3% | 0_1ins56;155_681del |
| 10 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_001282311.2 | 19.5% | 6.8% | 0_1ins56;155_732del |
| 11 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_005274716.3 | 19.5% | 6.8% | 0_1ins56;155_732del |
| 12 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_024452416.1 | 19% | 6.6% | 0_1ins56;155_753del |
| 13 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_024452415.1 | 18.9% | 6.6% | 0_1ins56;155_756del |
| 14 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XR_002958799.1 | 9.7% | 1_320del;531_2151del | |
| 15 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XR_002958798.1 | 9.5% | 1_378del;589_2209del | |
| 16 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XR_002958800.1 | 8.8% | (many diffs) | |
| 17 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XR_002958801.1 | 8.3% | (many diffs) | |
| 18 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XR_002958802.1 | 7.9% | (many diffs) | |
| 19 | human | 101929703 | LOC101929703 | uncharacterized LOC101929703 | XR_002958367.1 | 3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 276
- ORF length:
- 210
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGAG GTTGGCAGGC CCTCTCCGCA TTGTGGTCCT AGTCGTCAGT GTGGGTGTCA 121 CATGGATCGT GGTCAGCATC CTCCTGGGTG GGCCTGGCAG TGGCTTTCCT CGCATCCAGC 181 AACTCTTCAC CAGCTGGAGT GCAGTGGTGC AATCTCGGTT CACTGCAACC TCTACCTCCT 241 GGGTTCAAGC GATTCTAGTG CCCCAGCCTC CCGAGTACCC AACTTTCTTG TACAAAGTGG 301 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 361 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 421 AGCCGGTTTA TTAATATTCT CTACGACGCG TTAAGTCgac aatcaacctc tggattacaa 481 aatttgtgaa agatt