Construct: ORF TRCN0000473849
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015868.1_s317c1
- Derived from:
- ccsbBroadEn_07769
- DNA Barcode:
- ACTAGTTACCCCACAGTGTTATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WIF1 (11197)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473849
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11197 | WIF1 | WNT inhibitory factor 1 | NM_007191.5 | 99.7% | 99.7% | 177A>G;219A>G;496C>A |
| 2 | mouse | 24117 | Wif1 | Wnt inhibitory factor 1 | NM_011915.2 | 88.7% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1203
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccggaggagc gccttccctg ccgccgcgct ctggctctgg agcatcctcc 121 tgtgcctgct ggcactgcgg gcggaggccg ggccgccgca ggaggagagc ctgtacctat 181 ggatcgatgc tcaccaggca agagtactca taggatttga agaagatatc ctgattgttt 241 cggaggggaa aatggcacct tttacacatg atttcagaaa agcgcaacag agaatgccag 301 ctattcctgt caatatccat tccatgaatt ttacctggca agctgcaggg caggcagaat 361 acttctatga attcctgtcc ttgcgctccc tggataaagg catcatggca gatccaaccg 421 tcaatgtccc tctgctggga acagtgcctc acaaggcatc agttgttcaa gttggtttcc 481 catgtcttgg aaaacaggat ggggtggcag catttgaagt ggatgtgatt gttatgaatt 541 ctgaaggcaa caccattctc aaaacacctc aaaatgctat cttctttaaa acatgtcaac 601 aagctgagtg cccaggcggg tgccgaaatg gaggcttttg taatgaaaga cgcatctgcg 661 agtgtccTGA TGGGTTCCAC GGACCTCACT GTGAGAAAGC CCTTTGTACC CCACGATGTA 721 TGAATGGTGG ACTTTGTGTG ACTCCTGGTT TCTGCATCTG CCCACCTGGA TTCTATGGAG 781 TGAACTGTGA CAAAGCAAAC TGCTCAACCA CCTGCTTTAA TGGAGGGACC TGTTTCTACC 841 CTGGAAAATG TATTTGCCCT CCAGGACTAG AGGGAGAGCA GTGTGAAATC AGCAAATGCC 901 CACAACCCTG TCGAAATGGA GGTAAATGCA TTGGTAAAAG CAAATGTAAG TGTTCCAAAG 961 GTTACCAGGG AGACCTCTGT TCAAAGCCTG TCTGCGAGCC TGGCTGTGGT GCACATGGAA 1021 CCTGCCATGA ACCCAACAAA TGCCAATGTC AAGAAGGTTG GCATGGAAGA CACTGCAATA 1081 AAAGGTACGA AGCCAGCCTC ATACATGCCC TGAGGCCAGC AGGCGCCCAG CTCAGGCAGC 1141 ACACGCCTTC ACTTAAAAAG GCCGAGGAGC GGCGGGATCC ACCTGAATCC AATTACATCT 1201 GGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGAAC TAGTTACCCC ACAGTGTTAT TGACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt