Construct: ORF TRCN0000473862
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001585.1_s317c1
- Derived from:
- ccsbBroadEn_07878
- DNA Barcode:
- CCCTGGAGGACGTGGTAGCTCGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CTDNEP1 (23399)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473862
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23399 | CTDNEP1 | CTD nuclear envelope phosph... | NM_001143775.2 | 99.8% | 100% | 82C>T |
| 2 | human | 23399 | CTDNEP1 | CTD nuclear envelope phosph... | NM_015343.5 | 99.8% | 100% | 82C>T |
| 3 | mouse | 67181 | Ctdnep1 | CTD nuclear envelope phosph... | NM_026017.2 | 93.5% | 99.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 798
- ORF length:
- 732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gcggacgcag tgtctgctgg ggctgcgcac gttcgtggcc ttcgccgcca 121 agctctggag cttcttcatt taccttttgc ggaggcagat ccgcacggta attcagtacc 181 aaactgttcg atatgatatc ctccccttat ctcctgtgtc ccggaatcgg ctagcccagg 241 tgaagaggaa gatcctggtg ctggatctgg atgagacact tattcactcc caccatgatg 301 gggtcctGAG GCCCACAGTC CGGCCTGGTA CGCCTCCTGA CTTCATCCTC AAGGTGGTAA 361 TAGACAAACA TCCTGTCCGG TTTTTTGTAC ATAAGAGGCC CCATGTGGAT TTCTTCCTGG 421 AAGTGGTGAG CCAGTGGTAC GAGCTGGTGG TGTTTACAGC AAGCATGGAG ATCTATGGCT 481 CTGCTGTGGC AGATAAACTG GACAATAGCA GAAGCATTCT TAAGAGGAGA TATTACAGAC 541 AGCACTGCAC TTTGGAGTTG GGCAGCTACA TCAAGGACCT CTCTGTGGTC CACAGTGACC 601 TCTCCAGCAT TGTGATCCTG GATAACTCCC CAGGGGCTTA CAGGAGCCAT CCAGACAATG 661 CCATCCCCAT CAAATCCTGG TTCAGTGACC CCAGCGACAC AGCCCTTCTC AACCTGCTCC 721 CAATGCTGGA TGCCCTCAGG TTCACCGCTG ATGTTCGTTC CGTGCTGAGC CGAAACCTTC 781 ACCAACATCG GCTCTGGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 841 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 901 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCCTGGA GGACGTGGTA 961 GCTCGGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt