Construct: ORF TRCN0000473872
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016269.1_s317c1
- Derived from:
- ccsbBroadEn_10768
- DNA Barcode:
- ACAACCCCCTACAATCCATCCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRINP1 (1620)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473872
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1620 | BRINP1 | BMP/retinoic acid inducible... | NM_014618.3 | 41.4% | 40.8% | (many diffs) |
| 2 | mouse | 56710 | Brinp1 | bone morphogenic protein/re... | NM_019967.2 | 39.4% | 40.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1026
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ctggaggttt gttgagctcc tctacttcct gtttatatgg ggccgtatct 121 cagtgcagcc ctcccaccag gaaccagctg ggacagacca acatgtctcc aaggaatttg 181 attggctcat ttcagacagg gggcctttcc accactccag gagctaccta tcctttgtgg 241 aaagacaccg tcaaggattt acaaccagat ataaaatata cagggagttt gcccgttgga 301 aggtgaggaa cacagccatc gagaggagag atctggtccg ccatccagtg cccctcatgc 361 cggagtttca aaggagcatc cgcctgcttg gcaggagacc taccactcag cagttcatcg 421 ataccatcat caaaaagtac ggcacccacc tgctcatctc agccacattg ggaggggagg 481 aggctttgac catgtatatg gacaaaagtc gcctcgacag gaagtcaggg aatgccactc 541 aaagtgttga agctctgcac cagctcgcat catcctACTT TGTTGACCGT GATGGTACCA 601 TGAGGAGGCT TCATGAGATC CAGATATCAA CTGGAGCAAT CAAGGTCACA GAGACACGCA 661 CTGGGCCTCT GGGCTGTAAC AGTTATGACA ATCTGGACTC TGTGAGTTCC GTCCTTCTGC 721 AAAGCACGGA GAGCAAACTG CACCTTCAAG GTCTTCAGAT AATCTTTCCT CAGTATCTGC 781 AAGAGAAGTT TGTCCAGTCG GCCTTGAGCT ATATCATGTG CAATGGGGAG GGGGAGTACC 841 TGTGCCAGAA CAGCCAGTGT CGCTGCCAAT GTGCCGAGGA GTTTCCGCAG TGCAACTGCC 901 CCATCACGGA CATCCAGATC ATGGAGTACA CGCTGGCCAA CATGGCCAAG TCTTGGGCCG 961 AAGCTTATAA GGACCTGGAG AATTCAGGTA GAGAGTCTCA CTCAGTGCCA CTGCATGAGT 1021 GGCCTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1081 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1141 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACAACCCCC TACAATCCAT CCATAACGCG 1201 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt