Construct: ORF TRCN0000473873
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000879.1_s317c1
- Derived from:
- ccsbBroadEn_02288
- DNA Barcode:
- GTTAACAATACAGTTGTCTAATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNE3 (10008)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473873
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10008 | KCNE3 | potassium voltage-gated cha... | NM_005472.4 | 100% | 100% | |
| 2 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_017017047.1 | 100% | 100% | |
| 3 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_017017048.1 | 100% | 100% | |
| 4 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_017017049.1 | 100% | 100% | |
| 5 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_017017051.2 | 100% | 100% | |
| 6 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_017017052.1 | 100% | 100% | |
| 7 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_011544713.2 | 70% | 70% | 1_132del |
| 8 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | NM_001190869.1 | 86.7% | 93.2% | (many diffs) |
| 9 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | NM_001190870.1 | 86.7% | 93.2% | (many diffs) |
| 10 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | NM_001190871.2 | 86.7% | 93.2% | (many diffs) |
| 11 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | NM_001190950.1 | 86.7% | 93.2% | (many diffs) |
| 12 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | NM_020574.5 | 86.7% | 93.2% | (many diffs) |
| 13 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | XM_006508058.2 | 86.7% | 93.2% | (many diffs) |
| 14 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | XM_006508059.2 | 86.7% | 93.2% | (many diffs) |
| 15 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | XM_006508062.1 | 86.7% | 93.2% | (many diffs) |
| 16 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | XM_017322388.1 | 86.7% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 399
- ORF length:
- 309
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGAGACTAC CAATGGAACG GAGACCTGGT 121 ATGAGAGCCT GCATGCCGTG CTGAAGGCTC TAAATGCCAC TCTTCACAGC AATTTGCTCT 181 GCCGGCCAGG GCCAGGGCTG GGGCCAGACA ACCAGACTGA AGAGAGGCGG GCCAGCCTAC 241 CTGGCCGTGA TGACAACTCC TACATGTACA TTCTCTTTGT CATGTTTCTA TTTGCTGTAA 301 CTGTGGGCAG CCTCATCCTG GGATACACCC GCTCCCGCAA AGTGGACAAG CGTAGTGACC 361 CCTATCATGT GTATATCAAG AACCGTGTGT CTATGATCTA CCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGAGTTAAC AATACAGTTG TCTAATCAAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt