Construct: ORF TRCN0000473888
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018924.3_s317c1
- Derived from:
- ccsbBroadEn_15054
- DNA Barcode:
- ACACCGGTTAATTCCCCAGGGAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PIK3R4 (30849)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473888
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30849 | PIK3R4 | phosphoinositide-3-kinase r... | NM_014602.3 | 99.5% | 18% | (many diffs) |
| 2 | mouse | 75669 | Pik3r4 | phosphoinositide-3-kinase r... | NM_001081309.1 | 85.3% | 17.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 807
- ORF length:
- 738
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaaatcag cttgctggca ttgctccctc ccagatcctt tctgtagaga 121 gttatttttc agatattcat gactttgaat atgataaaag cctggggagt actcggtttt 181 ttaaagttgc tcgagccaag caccgagaag gcctggtcgt tgtgaaggtt tttgcaattc 241 aggatcccac attgccttta accagctata aacaagagct ggaggaactg aaaatcaggc 301 ttaattctgc acagaattgt ctacctttcc agaaagcatc agaaaaagca tctgagaaag 361 cagctatgct ctttaggcag tatgtgcgag acaatctcta tgatcgcatc agtacccgtc 421 cattcttgaa taacattgag aagcgctgga ttgctttcca gatcctgaca gctgtggacc 481 aagcacacaa atctggagtt cgtcatgggg acatcaagac tgagaatgtg atggtcacca 541 gttggaattg ggttcttcta actgattttg ccagttttaa gcccacttat cttccagaag 601 acaacccggc agatttcaat tatttctttg acacatcacg gaggagaact tgctatattg 661 ctcctgaacg ttttgttgat ggtgggatgt ttgccactga gttagaatat atgagagatc 721 cttcaactcc gcttgtagac ttaaatagca atcagagaac aagaggagag ttgaagagag 781 caatggacat cttttcagca gttgtgtgat agctgagctt tttacagaag tgtaccatta 841 tttgatctct ctcaactttt ggcttataga aatggacatt ttttccctga acaagtgcta 901 aataaaattg aagatcacag tatcagagaa ttggtaactc agatgattca ccgtgagcca 961 gataaacgtt tagaggcaga agattactta aaacagcagc gtggcaatgc ctttcctgaa 1021 atattttaca cttttcttca gccctacatg gcccagtttg ccaaggaaac gtttctttct 1081 gcagatgagc gtattctggt tatacggaag gatttgggca acattattca caatctctgt 1141 ggacatgatc tgccagaaaa agccgaagga gagcctaagg aaaatgggct ggttatcttg 1201 gtatctgtta taacatcctg cctacagacc cttaaatact gtgattccaa actagctgct 1261 ttggaactga ttcttcattt ggctccaaga ttaagtgttg aaatcctttt ggatcgtatt 1321 actccatatc ttttgcattt cagcaatgac tctgttccta gggtgagggc tgaagccttg 1381 aggacgttga ccaaagttct tgctctcgtc aaagaggttc ctcgtaatga tatcaatatt 1441 tatccggaat acattctgcc aggcatagcc cacttagccc aagatgatgc tactatcgtt 1501 agactagcct atgctgaaaa catagctctg ctggcagaaa cagctctgag attcctggaa 1561 ttagtacagt taaaaaatct taatatggaa aatgacccca ataatgaaga aatagatgag 1621 gttacacatc caaatggaaa ttatgacaca gagctccaag ccttacatga aatggtccag 1681 cagaaagttg ttactttgct aagtgaccct gaaaatattg taaaacaaac cttgatggaa 1741 aatggaataa cacggctgtg tgtattcttt ggacgtcaga aagccaacga tgttttgttg 1801 tcccacatga ttactttcct aaatgataag aatgattggc atctacgtgg agcatttttt 1861 gatagtatag ttggtgttgc tgcctatgtt ggctggcaaa gctcctcaat tctcaagcct 1921 ctgctgcaac aaggtcttag tgatgctgag gaatttgtca ttgtgaaagc tctttatgcc 1981 cttacttgta tgtgccagtt aggactgcta caaaaacccc atgtttacga atttgccagt 2041 gatattgccc ccttcctgtg tcatcccaat ttatggatac gttatggtgc cgtgggattt 2101 atcacagtgg tagctcgtca aataagtaca gctgatgtct actgtaaact gatgccttat 2161 cttgacccat atattaccca accaataata cagattgaaa gaaaacttgt tctgctcagt 2221 gttttaaagg aaccagtaag tcgttctata tttgattatg ctttgaggtc taaagatatt 2281 actagcttgt tcagacatct tcacatgcgt cagaagaaac gaaatggttc tcttcccgac 2341 tgccctccgc cagaggatcc tgccatagca cagcttctga agaagttgct ctcacaggga 2401 atgacagagg aagaggaaga caaacttctg gcactgaaag acttcatgat gaaatctaat 2461 aaagcaaagg ccaatatagt ggaccagagc catcttcatg atagtagtca gaaaggtgta 2521 attgacttgg cagctttagg cataactggg agacaagttg atcttgttaa aaccaaacaa 2581 gaaccagatg acaaacgggc cagaaaacat gtaaaacaag actcaaatgt aaatgaagaa 2641 tggaaaagca tgtttgggtc actggaccca ccaaacatgc cacaggccct acctaaaggg 2701 agtgatcagg aggtgattca gactgggaaa cctcctcgtt ccgagtcctc tgctggcatt 2761 tgtgtccctt tgtcaacttc ttcacaggtt ccagaagtga caactgtcca aaataaaaaa 2821 ccagtaatac cggttttaag tagtacaatc ttaccatcca cctatcagat tcgaattaca 2881 acttgtaaaa ctgaacttca gcaactcatc cagcaaaagc gggagcagtg caatgctgag 2941 agaatagcta agcagatgat ggaaaatgct gaatgggaga gtaaaccacc accacctgga 3001 tggcgtccta aagggctgtt agttgcccat cttcatgagc ataaatctgc tgtgaatcga 3061 attagagtct ctgatgaaca ctcacttttt gcaacatgtt caaatgatgg cacagtgaaa 3121 atctggaaca gtcaaaagat ggaggggaag accaccacta ccagatctat tcttacatac 3181 agccgaattg gaggacgagt caagacgctc acattctgcc aaggctccca ctatttagcc 3241 atagcatctg ataatggtgc tgtccagctt cttggaattg aggcttctaa gctgcccaag 3301 tctcctaaaa tccatcctct acaaagcaga attctagatc agaaggagga cgggtgtgtt 3361 gtggatatgc atcacttcac ttctggagca cagtctgttc tgcnntatgc cactgtgaat 3421 ggctctctgg tnggctggac cntagggtct caaagcaatg cgtggactta aaagcatgat 3481 ttaaagtcgg gcctcatcac ttcctttgct gtggacatcc acccaatgct ggctctgcat 3541 tggtacaagc agtggtacca tggcttgttg ggacatgagg ttccagttgc caatttcaag 3601 tcactgtcat ccttccaggg ctcgaatcag acgcctctca atgcaccctc tgtatcagtc 3661 ctgggtgatt gcagctgttc agggcaacaa cgaagtgtcc atgtgggaca tggagactgg 3721 tgacagaaga tttactcTCT GGGCCAGCAG TGCACCACCA CTTTCTGAAT TACAGCCTTC 3781 TCCTCATAGC GTCCATGGTA TCTACTGTAG TCCTGCAGAT GGAAATCCTA TCCTACTAAC 3841 AGCTGGCTCA GATATGAAAA TAAGGTTTTG GGACTTGGCT TACCCAGAAA GGTCCTATGT 3901 TGTTGCAGGA AGTACTAGTT CCCCATCTGT GTCCTACTAC AGGAAAATAA TTGAAGGCAC 3961 TGAAGTTGTC CAGGAAATTC AGAATAAGCA GAAAGTAGGA CCAAGTGATG ACACCCCTCG 4021 AAGGGGCCCA GAGTCCCTGC CCGTGGGACA TCATGACATC ATCACTGATG TCGCCACATT 4081 CCAGACCACA CAGGGCTTCA TCGTAACTGC TTCTAGAGAT GGGATTGTGA AGGTGTGGAA 4141 ATTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT 4201 CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG 4261 GCTTTATATA TCTTGTGGAA AGGACGAACA CCGGTTAATT CCCCAGGGAG AACGCGTTAA 4321 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t