Construct: ORF TRCN0000473893
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003104.1_s317c1
- Derived from:
- ccsbBroadEn_08559
- DNA Barcode:
- TAACCTCGGTCATTCTTTGTGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D22B (55633)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473893
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55633 | TBC1D22B | TBC1 domain family member 22B | NM_017772.4 | 99.8% | 100% | 573A>G;771C>T |
| 2 | human | 55633 | TBC1D22B | TBC1 domain family member 22B | XM_011514738.3 | 92.3% | 90.9% | (many diffs) |
| 3 | human | 55633 | TBC1D22B | TBC1 domain family member 22B | XM_011514739.2 | 76.8% | 76.8% | (many diffs) |
| 4 | human | 55633 | TBC1D22B | TBC1 domain family member 22B | XR_926270.3 | 73.7% | (many diffs) | |
| 5 | human | 55633 | TBC1D22B | TBC1 domain family member 22B | XR_427833.2 | 43.2% | (many diffs) | |
| 6 | human | 55633 | TBC1D22B | TBC1 domain family member 22B | NR_130108.2 | 42.9% | (many diffs) | |
| 7 | human | 55633 | TBC1D22B | TBC1 domain family member 22B | XR_241906.2 | 40.3% | (many diffs) | |
| 8 | mouse | 381085 | Tbc1d22b | TBC1 domain family, member 22B | NM_198647.1 | 89.5% | 98.8% | (many diffs) |
| 9 | mouse | 381085 | Tbc1d22b | TBC1 domain family, member 22B | XM_006524488.1 | 86.4% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1584
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgctgag aacagcaagc agttttggaa gaggagcgct aagctgccgg 121 ggagcattca gcctgtatat ggagcacagc atcctcctct tgacccacgg ctcaccaaaa 181 atttcattaa agaacgatca aaagtcaaca cagttcctct gaagaataag aaggcctcca 241 gttttcatga gtttgcacgg aataccagtg atgcttggga cattggcgat gatgaggaag 301 aggacttttc ctcaccttct ttccaaactc tgaactcaaa agttgctttg gcaactgcag 361 cccaagttct agaaaaccac agcaagctga gagtaaaacc agaacggtcc cagtcaacga 421 catcggacgt ccctgccaac tacaaggtca taaagtccag cagtgatgcc cagctgtcca 481 gaaactctag tgatacatgc ctgaggaacc cactccacaa acagcaatca ctccctctcc 541 ggcccatcat ccccctcgtt gcccggatct cggatcagaa cgcttctggg gcccccccaa 601 tgactgtccg ggagaaaacc cgcctagaaa aattccgtca gcttctctcc agccagaaca 661 ctgacttaga tgaactgagg aagtgtagct ggccaggggt tcccagggag gttcgaccta 721 taacctggag actcctgtcg ggctatctcc cagcaaacac tgagaggagg aagttgaccc 781 tgcagcggaa gcgggaggaa tattttggct tcattgaaca gtattatgac tctcgaaatg 841 aggaacatca ccaggatacc tacagacaga ttcacattga cattccaagg acgaatcctc 901 tcattccgtt gttccagcaa ccacttgtac aggagatctt tgaaagaatt ctatttattt 961 gggccatccg ccaccctgcc agtgggtatg tccagggaat taatgacctg gtcactccat 1021 tctttgtcgt cttcctctca gaatatgtgg aagaggatgt ggagaacttt gacgtgacca 1081 acttgtctca agacatgctg cgaagcattg aggctgacag cTTTTGGTGC ATGAGCAAGC 1141 TGCTGGATGG AATCCAGGAT AACTACACCT TTGCACAACC AGGAATCCAG AAGAAGGTGA 1201 AGGCACTGGA AGAGCTTGTC AGCCGGATTG ATGAGCAGGT ACATAATCAC TTCAGGAGGT 1261 ACGAGGTAGA ATACCTGCAG TTTGCCTTCC GCTGGATGAA CAACCTGCTT ATGCGGGAGC 1321 TTCCTCTTCG CTGCACCATC CGCCTGTGGG ACACATATCA GTCTGAACCA GAAGGGTTCT 1381 CCCACTTTCA TCTCTACGTG TGTGCAGCCT TCTTGATCAA GTGGAGGAAA GAGATCTTGG 1441 ATGAGGAGGA TTTTCAGGGT CTCCTCATGC TGCTACAGAA CCTACCTACA ATACACTGGG 1501 GCAACGAAGA AATTGGGCTG CTTCTCGCCG AGGCATACAG ACTCAAGTAC ATGTTTGCCG 1561 ATGCCCCAAA TCACTACCGC CGATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1621 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1681 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AACCTCGGTC 1741 ATTCTTTGTG CAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1801 att