Construct: ORF TRCN0000473913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006837.1_s317c1
- Derived from:
- ccsbBroadEn_07078
- DNA Barcode:
- AACTCCTTGGCACCGGACACGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN6 (7105)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473913
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7105 | TSPAN6 | tetraspanin 6 | NM_003270.4 | 99.8% | 100% | 381G>A |
| 2 | human | 7105 | TSPAN6 | tetraspanin 6 | NM_001278740.2 | 63.9% | 64% | 0_1ins264;117G>A |
| 3 | human | 7105 | TSPAN6 | tetraspanin 6 | NM_001278741.1 | 63.9% | 64% | 0_1ins264;117G>A |
| 4 | human | 7105 | TSPAN6 | tetraspanin 6 | XM_011531018.1 | 63.9% | 64% | 0_1ins264;117G>A |
| 5 | human | 7105 | TSPAN6 | tetraspanin 6 | NM_001278742.1 | 55.3% | 55.5% | (many diffs) |
| 6 | human | 7105 | TSPAN6 | tetraspanin 6 | NM_001278743.1 | 52.5% | 52.6% | 0_1ins264;117G>A;319_320ins84 |
| 7 | mouse | 56496 | Tspan6 | tetraspanin 6 | NM_019656.3 | 87.6% | 93% | (many diffs) |
| 8 | mouse | 56496 | Tspan6 | tetraspanin 6 | XM_017318578.1 | 56% | 57.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 801
- ORF length:
- 735
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtccccgtct cggagactgc agactaaacc agtcattact tgtttcaaga 121 gcgttctgct aatctacact tttattttct ggatcactgg cgttatcctt cttgcagttg 181 gcatttgggg caaggtgagc ctggagaatt acttttctct tttaaatgag aaggccacca 241 atgtcccctt cgtgctcatt gctactggta ccgtcattat tcttttgggc acctttggtt 301 gttttgctac ctgccgagcT TCTGCATGGA TGCTAAAACT GTATGCAATG TTTCTGACTC 361 TCGTTTTTTT GGTCGAACTG GTCGCTGCCA TCGTAGGATT TGTTTTCAGA CATGAGATTA 421 AGAACAGCTT TAAGAATAAT TATGAAAAGG CTTTGAAGCA GTATAACTCT ACAGGAGATT 481 ATAGAAGCCA TGCAGTAGAC AAGATCCAAA ATACGTTGCA TTGTTGTGGT GTCACCGATT 541 ATAGAGATTG GACAGATACT AATTATTACT CAGAAAAAGG ATTTCCTAAG AGTTGCTGTA 601 AACTTGAAGA TTGTACTCCA CAGAGAGATG CAGACAAAGT AAACAATGAA GGTTGTTTTA 661 TAAAGGTGAT GACCATTATA GAGTCAGAAA TGGGAGTCGT TGCAGGAATT TCCTTTGGAG 721 TTGCTTGCTT CCAACTGATT GGAATCTTTC TCGCCTACTG CCTCTCTCGT GCCATAACAA 781 ATAACCAGTA TGAGATAGTG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 841 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 901 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAACT CCTTGGCACC 961 GGACACGGCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt