Construct: ORF TRCN0000473914
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003967.1_s317c1
- Derived from:
- ccsbBroadEn_06865
- DNA Barcode:
- TTATGTAATCTCTGGTTCAGTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RIT2 (6014)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473914
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6014 | RIT2 | Ras like without CAAX 2 | NM_002930.4 | 99.8% | 99.5% | 598A>T |
| 2 | human | 6014 | RIT2 | Ras like without CAAX 2 | NM_001272077.1 | 68.6% | 66.5% | (many diffs) |
| 3 | mouse | 19762 | Rit2 | Ras-like without CAAX 2 | NM_009065.2 | 86.3% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggtagaaaat gaagccagct gctccccggg cagcgcatca ggcgggtcca 121 gagagtacaa ggtggtaatg ctgggagcag ggggagttgg taaaagcgca atgacaatgc 181 agtttattag tcatcagttc cctgattatc atgacccTAC TATAGAAGAT GCTTATAAGA 241 CCCAGGTCAG GATTGACAAT GAGCCAGCTT ACTTGGACAT CTTGGACACT GCTGGCCAGG 301 CAGAATTCAC AGCCATGCGG GAGCAGTACA TGCGAGGTGG GGAAGGCTTC ATCATCTGCT 361 ACTCCGTCAC TGACCGTCAA TCATTTCAGG AGGCTGCCAA GTTTAAAGAG CTCATTTTTC 421 AGGTCCGCCA CACCTATGAA ATTCCCCTGG TGCTGGTGGG TAACAAAATT GATCTGGAAC 481 AGTTCCGCCA GGTTTCTACA GAAGAAGGCT TGAGTCTTGC CCAAGAATAT AATTGTGGTT 541 TTTTTGAGAC CTCTGCAGCC CTCAGATTCT GTATTGATGA TGCTTTTCAT GGCTTAGTGA 601 GGGAAATTCG CAAGAAGGAG TCCATGCCAT CCTTGATGGA AAAGAAACTG AAGAGAAAAG 661 ACTGCCTGTG GAAGAAGCTC AAAGGTTCTT TGAAGAAGAA GAGAGAAAAT ATGACATGCC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GATTATGTAA TCTCTGGTTC AGTATAACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt