Construct: ORF TRCN0000473921
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006663.1_s317c1
- Derived from:
- ccsbBroadEn_06525
- DNA Barcode:
- TAAAAGACAAGCGTTTTCTGTTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIPA (3988)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473921
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3988 | LIPA | lipase A, lysosomal acid type | NM_000235.4 | 99.8% | 99.4% | 46A>C;85G>T |
| 2 | human | 3988 | LIPA | lipase A, lysosomal acid type | NM_001127605.3 | 99.8% | 99.4% | 46A>C;85G>T |
| 3 | human | 3988 | LIPA | lipase A, lysosomal acid type | XM_024448023.1 | 99.8% | 99.4% | 46A>C;85G>T |
| 4 | human | 3988 | LIPA | lipase A, lysosomal acid type | NM_001288979.1 | 70.9% | 70.9% | 0_1ins348 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1263
- ORF length:
- 1197
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa aatgcggttc ttggggttgg tggtctgttt ggttctctgg cccctgcatt 121 ctgaggggtc tggagggaaa ctgacagctt tggatcctga aacaaacatg aatgtgagtg 181 aaattatctc ttactgggga ttccctagtg aggaatacct agttgagaca gaagatggat 241 atattctgtg ccttaaccga attcctcatg ggaggaagaa ccattctgac aaaggtccca 301 aaccagttgt cttcctgcaa catggcttgc tggcagattc tagtaactgg gtcacaaacc 361 ttgccaacag cagcctgggc ttcattcttg ctgatgctgg ttttgacgtg tggatgggca 421 acagcagagg aaatacctgg tctcggaaac ataagacact ctcagtttct caggatgaat 481 tctgggcttt cagttatgat gagatggcaa aatatgacct accagcttcc attaacttca 541 ttctgaataa aactggccaa gaacaagtgt attatgtggg tcattctcaa ggcaccacta 601 taggttttat agcattttca cagatccctg agctggctaa aaggattaaa atgttttttg 661 ccctgggtcc tgtggcttcc gtcgccttct gtactagccc tatggccaaa ttaggacgat 721 taccagatca tctcattaag gacttatttg gagacaaaga atttcttccc cagagtgcgt 781 ttttgaagtg gctgggtacc cacgtttgca ctcatgtcat actgaaggag ctctgtggaa 841 ATCTCTGTTT TCTTCTGTGT GGATTTAATG AGAGAAATTT AAATATGTCT AGAGTGGATG 901 TATATACAAC ACATTCTCCT GCTGGAACTT CTGTGCAAAA CATGTTACAC TGGAGCCAGG 961 CTGTTAAATT CCAAAAGTTT CAAGCCTTTG ACTGGGGAAG CAGTGCCAAG AATTATTTTC 1021 ATTACAACCA GAGTTATCCT CCCACATACA ATGTGAAGGA CATGCTTGTG CCGACTGCAG 1081 TCTGGAGCGG GGGTCACGAC TGGCTTGCAG ATGTCTACGA CGTCAATATC TTACTGACTC 1141 AGATCACCAA CTTGGTGTTC CATGAGAGCA TTCCGGAATG GGAGCATCTT GACTTCATTT 1201 GGGGCCTGGA TGCCCCTTGG AGGCTTTATA ATAAAATTAT TAATCTAATG AGGAAATATC 1261 AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1321 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1381 GGCTTTATAT ATCTTGTGGA AAGGACGATA AAAGACAAGC GTTTTCTGTT GTACGCGTTA 1441 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt