Construct: ORF TRCN0000473922

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011371.1_s317c1
Derived from:
ccsbBroadEn_02651
DNA Barcode:
GCCCGTTACTCTTGTGCTATACGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RASSF8 (11228)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473922
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11228 RASSF8 Ras association domain fami... NM_007211.5 100% 100%
2 human 11228 RASSF8 Ras association domain fami... NM_001164746.2 92.3% 90.6% (many diffs)
3 human 11228 RASSF8 Ras association domain fami... NM_001164747.2 92.3% 90.6% (many diffs)
4 human 11228 RASSF8 Ras association domain fami... NM_001164748.2 92.3% 90.6% (many diffs)
5 human 11228 RASSF8 Ras association domain fami... XM_006719027.2 92.3% 90.6% (many diffs)
6 human 11228 RASSF8 Ras association domain fami... XM_006719028.1 92.3% 90.6% (many diffs)
7 human 11228 RASSF8 Ras association domain fami... XM_017018746.2 92.3% 90.6% (many diffs)
8 human 11228 RASSF8 Ras association domain fami... XM_024448818.1 92.3% 90.6% (many diffs)
9 human 11228 RASSF8 Ras association domain fami... XM_024448819.1 92.3% 90.6% (many diffs)
10 human 11228 RASSF8 Ras association domain fami... XM_024448820.1 92.3% 90.6% (many diffs)
11 human 11228 RASSF8 Ras association domain fami... XM_024448821.1 92.3% 90.6% (many diffs)
12 human 11228 RASSF8 Ras association domain fami... XM_024448822.1 92.3% 90.6% (many diffs)
13 mouse 71323 Rassf8 Ras association (RalGDS/AF-... NM_027760.2 76% 82.5% (many diffs)
14 mouse 71323 Rassf8 Ras association (RalGDS/AF-... XM_011241598.1 76% 82.5% (many diffs)
15 mouse 71323 Rassf8 Ras association (RalGDS/AF-... XM_011241599.1 76% 82.5% (many diffs)
16 mouse 71323 Rassf8 Ras association (RalGDS/AF-... XM_011241600.2 76% 82.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1242
ORF length:
1176
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga acttaaagta tgggtggatg gagttcagag gattgtttgt ggagtcactg
121aagtcacaac ttgccaggag gttgtcatag ccttagctca agcaataggt cgaactggaa
181ggtacaccct tatagagaaa tggagagata ctgaaagaca cttagcacct catgaaaatc
241ctatcatatc cttaaacaaa tgggggcagt atgctagtga tgtgcagctc attctacgac
301gaactgggcc gtctctcagt gagcgaccca cttcagacag tgtggctcga attcctgaaa
361gaactttata caggcagagt ctgcccccct tagctaaact gaggcctcag attgacaaat
421caatcaaaag gagggaaccg aaaaggaaat cactgacatt tacaggaggt gccaaaggat
481taatggacat ttttggaaaa ggtaaagaaa ctgagtttaa gcaaaaggtg ctgaataact
541gcaaaacaac agcagatgag ttgaagaagc taatccgtct gcagacagag aagcttcaat
601ccattgagaa acagctggaa tctaatgaaa tagaaataag attttgggag caaaagtata
661attccaacct tgaagaggaa attgtccgtc tagagCAAAA GATCAAAAGA AACGATGTAG
721AAATTGAGGA GGAAGAATTC TGGGAAAATG AATTACAGAT TGAACAGGAA AATGAAAAAC
781AGCTGAAGGA TCAACTTCAA GAAATAAGAC AGAAAATAAC AGAATGTGAA AACAAATTAA
841AGGACTATTT GGCACAGATC CGGACTATGG AAAGTGGTCT TGAAGCAGAA AAATTGCAAC
901GGGAAGTTCA AGAGGCACAG GTCAATGAGG AAGAGGTTAA AGGAAAGATC GGTAAGGTCA
961AAGGGGAGAT TGACATTCAA GGCCAGCAGA GTCTGAGGTT GGAAAATGGC ATCAAAGCTG
1021TGGAAAGATC TCTTGGACAA GCCACCAAAC GCTTACAGGA CAAAGAACAG GAACTGGAGC
1081AGTTGACTAA GGAGTTGCGG CAAGTCAATC TCCAGCAGTT CATCCAGCAG ACAGGGACAA
1141AAGTTACCGT TTTGCCAGCG GAGCCCATTG AAATAGAGGC CTCACATGCA GACATTGAAA
1201GGGGGATCAT CATTCTTTCT GATAAGCAGG AGTGTAAAGA TTACCCAACT TTCTTGTACA
1261AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1321AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1381AGGACGAGCC CGTTACTCTT GTGCTATACG CACGCGTTAA GTCgacaatc aacctctgga
1441ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)