Construct: ORF TRCN0000473922
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011371.1_s317c1
- Derived from:
- ccsbBroadEn_02651
- DNA Barcode:
- GCCCGTTACTCTTGTGCTATACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASSF8 (11228)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473922
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11228 | RASSF8 | Ras association domain fami... | NM_007211.5 | 100% | 100% | |
| 2 | human | 11228 | RASSF8 | Ras association domain fami... | NM_001164746.2 | 92.3% | 90.6% | (many diffs) |
| 3 | human | 11228 | RASSF8 | Ras association domain fami... | NM_001164747.2 | 92.3% | 90.6% | (many diffs) |
| 4 | human | 11228 | RASSF8 | Ras association domain fami... | NM_001164748.2 | 92.3% | 90.6% | (many diffs) |
| 5 | human | 11228 | RASSF8 | Ras association domain fami... | XM_006719027.2 | 92.3% | 90.6% | (many diffs) |
| 6 | human | 11228 | RASSF8 | Ras association domain fami... | XM_006719028.1 | 92.3% | 90.6% | (many diffs) |
| 7 | human | 11228 | RASSF8 | Ras association domain fami... | XM_017018746.2 | 92.3% | 90.6% | (many diffs) |
| 8 | human | 11228 | RASSF8 | Ras association domain fami... | XM_024448818.1 | 92.3% | 90.6% | (many diffs) |
| 9 | human | 11228 | RASSF8 | Ras association domain fami... | XM_024448819.1 | 92.3% | 90.6% | (many diffs) |
| 10 | human | 11228 | RASSF8 | Ras association domain fami... | XM_024448820.1 | 92.3% | 90.6% | (many diffs) |
| 11 | human | 11228 | RASSF8 | Ras association domain fami... | XM_024448821.1 | 92.3% | 90.6% | (many diffs) |
| 12 | human | 11228 | RASSF8 | Ras association domain fami... | XM_024448822.1 | 92.3% | 90.6% | (many diffs) |
| 13 | mouse | 71323 | Rassf8 | Ras association (RalGDS/AF-... | NM_027760.2 | 76% | 82.5% | (many diffs) |
| 14 | mouse | 71323 | Rassf8 | Ras association (RalGDS/AF-... | XM_011241598.1 | 76% | 82.5% | (many diffs) |
| 15 | mouse | 71323 | Rassf8 | Ras association (RalGDS/AF-... | XM_011241599.1 | 76% | 82.5% | (many diffs) |
| 16 | mouse | 71323 | Rassf8 | Ras association (RalGDS/AF-... | XM_011241600.2 | 76% | 82.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1242
- ORF length:
- 1176
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga acttaaagta tgggtggatg gagttcagag gattgtttgt ggagtcactg 121 aagtcacaac ttgccaggag gttgtcatag ccttagctca agcaataggt cgaactggaa 181 ggtacaccct tatagagaaa tggagagata ctgaaagaca cttagcacct catgaaaatc 241 ctatcatatc cttaaacaaa tgggggcagt atgctagtga tgtgcagctc attctacgac 301 gaactgggcc gtctctcagt gagcgaccca cttcagacag tgtggctcga attcctgaaa 361 gaactttata caggcagagt ctgcccccct tagctaaact gaggcctcag attgacaaat 421 caatcaaaag gagggaaccg aaaaggaaat cactgacatt tacaggaggt gccaaaggat 481 taatggacat ttttggaaaa ggtaaagaaa ctgagtttaa gcaaaaggtg ctgaataact 541 gcaaaacaac agcagatgag ttgaagaagc taatccgtct gcagacagag aagcttcaat 601 ccattgagaa acagctggaa tctaatgaaa tagaaataag attttgggag caaaagtata 661 attccaacct tgaagaggaa attgtccgtc tagagCAAAA GATCAAAAGA AACGATGTAG 721 AAATTGAGGA GGAAGAATTC TGGGAAAATG AATTACAGAT TGAACAGGAA AATGAAAAAC 781 AGCTGAAGGA TCAACTTCAA GAAATAAGAC AGAAAATAAC AGAATGTGAA AACAAATTAA 841 AGGACTATTT GGCACAGATC CGGACTATGG AAAGTGGTCT TGAAGCAGAA AAATTGCAAC 901 GGGAAGTTCA AGAGGCACAG GTCAATGAGG AAGAGGTTAA AGGAAAGATC GGTAAGGTCA 961 AAGGGGAGAT TGACATTCAA GGCCAGCAGA GTCTGAGGTT GGAAAATGGC ATCAAAGCTG 1021 TGGAAAGATC TCTTGGACAA GCCACCAAAC GCTTACAGGA CAAAGAACAG GAACTGGAGC 1081 AGTTGACTAA GGAGTTGCGG CAAGTCAATC TCCAGCAGTT CATCCAGCAG ACAGGGACAA 1141 AAGTTACCGT TTTGCCAGCG GAGCCCATTG AAATAGAGGC CTCACATGCA GACATTGAAA 1201 GGGGGATCAT CATTCTTTCT GATAAGCAGG AGTGTAAAGA TTACCCAACT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGAGCC CGTTACTCTT GTGCTATACG CACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t