Construct: ORF TRCN0000473937

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006669.1_s317c1
Derived from:
ccsbBroadEn_08303
DNA Barcode:
CCAAAACGATTCATATGGTGAGAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHMP5 (51510)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473937
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51510 CHMP5 charged multivesicular body... NM_016410.6 99.8% 100% 603A>G
2 human 51510 CHMP5 charged multivesicular body... NM_001195536.1 77.7% 75.7% (many diffs)
3 mouse 76959 Chmp5 charged multivesicular body... NM_029814.1 87.6% 98.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
723
ORF length:
657
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa ccgactcttc gggaaagcga aacccaaggc tccgccgccc agcctgactg
121actgcattgg cacggtggac agtagagcag aatccattga caagaagatt tctcgattgg
181atgctgagct agtgaagtat aaggatcaga tcaagaagat gagagagggT CCTGCAAAGA
241ATATGGTCAA GCAGAAAGCC TTGCGAGTTT TAAAGCAAAA GAGGATGTAT GAGCAGCAGC
301GGGACAATCT TGCCCAACAG TCATTCAACA TGGAACAAGC CAATTATACC ATCCAGTCTT
361TGAAGGACAC CAAGACCACG GTTGATGCTA TGAAACTGGG AGTAAAGGAA ATGAAGAAGG
421CATACAAGCA AGTGAAGATC GACCAGATTG AGGATTTACA AGACCAGCTA GAGGATATGA
481TGGAAGATGC AAATGAAATC CAAGAAGCAC TGAGTCGCAG TTATGGCACC CCAGAACTGG
541ATGAAGATGA TTTAGAAGCA GAGTTGGATG CACTAGGTGA TGAGCTTCTG GCTGATGAAG
601ACAGTTCTTA TTTGGATGAG GCAGCATCTG CACCTGCAAT TCCAGAAGGT GTTCCCACTG
661ATACAAAGAA CAAGGATGGA GTTCTGGTGG ATGAATTTGG ATTGCCACAG ATCCCTGCTT
721CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
781TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
841GGCTTTATAT ATCTTGTGGA AAGGACGACC AAAACGATTC ATATGGTGAG AAACGCGTTA
901AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)