Construct: ORF TRCN0000473938
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000537.1_s317c1
- Derived from:
- ccsbBroadEn_07369
- DNA Barcode:
- AGAGGTTTGCTATCCCGTCCTCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EBAG9 (9166)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473938
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9166 | EBAG9 | estrogen receptor binding s... | NM_001278938.1 | 99.8% | 99.5% | 547A>G |
| 2 | human | 9166 | EBAG9 | estrogen receptor binding s... | NM_004215.5 | 99.8% | 99.5% | 547A>G |
| 3 | human | 9166 | EBAG9 | estrogen receptor binding s... | NM_198120.2 | 99.8% | 99.5% | 547A>G |
| 4 | human | 9166 | EBAG9 | estrogen receptor binding s... | XM_017013960.1 | 99.8% | 99.5% | 547A>G |
| 5 | mouse | 55960 | Ebag9 | estrogen receptor-binding f... | NM_019480.4 | 90.9% | 97.1% | (many diffs) |
| 6 | mouse | 55960 | Ebag9 | estrogen receptor-binding f... | XM_006521193.2 | 90.9% | 97.1% | (many diffs) |
| 7 | mouse | 55960 | Ebag9 | estrogen receptor-binding f... | XM_006521194.3 | 90.9% | 97.1% | (many diffs) |
| 8 | mouse | 55960 | Ebag9 | estrogen receptor-binding f... | XM_006521195.3 | 90.9% | 97.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggtatggc catcacccag tttcggttat ttaaattttg tacctgccta gcaacagtat 121 tctcattcct aaagagatta atatgcagat ctggcagagg acggaaatta agtggAGACC 181 AAATAACTTT GCCAACTACA GTTGATTATT CATCAGTTCC TAAGCAGACA GATGTTGAAG 241 AGTGGACTTC CTGGGATGAA GATGCACCCA CCAGTGTAAA GATCGAAGGA GGGAATGGGA 301 ATGTGGCAAC ACAACAAAAT TCTTTGGAAC AACTGGAACC TGACTATTTT AAGGACATGA 361 CACCAACTAT TAGGAAAACT CAGAAAATTG TTATTAAGAA GAGAGAACCA TTGAATTTTG 421 GCATCCCAGA TGGGAGCACA GGTTTCTCTA GTAGATTAGC AGCTACACAA GATCTGCCTT 481 TTATTCATCA GTCTTCTGAA TTAGGTGACT TAGATACCTG GCAGGAAAAT ACCAATGCAT 541 GGGAAGAAGA AGAAGATGCA GCCTGGCAAG CAGAAGAAGT TCTGAGACAG CAGAAACTAG 601 CAGACAGAGA AGAGAGAGCA GCCGAACAAC AAAGGAAGAA AATGGAAAAG GAAGCACAAC 661 GGCTAATGAA GAAGGAACAA AACAAAATTG GTGTGAAACT TTCATACCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA AGAGGTTTGC TATCCCGTCC TCCGACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt