Transcript: Human NM_004215.5

Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-06-08
Taxon:
Homo sapiens (human)
Gene:
EBAG9 (9166)
Length:
2383
CDS:
305..946

Additional Resources:

NCBI RefSeq record:
NM_004215.5
NBCI Gene record:
EBAG9 (9166)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004215.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000061085 CCCACCAGTGTAAAGATCGAA pLKO.1 506 CDS 100% 3.000 4.200 N EBAG9 n/a
2 TRCN0000061087 CGGAAATTAAGTGGAGACCAA pLKO.1 401 CDS 100% 2.640 3.696 N EBAG9 n/a
3 TRCN0000061083 GCCTAGCAACAGTATTCTCAT pLKO.1 345 CDS 100% 4.950 3.960 N EBAG9 n/a
4 TRCN0000300000 GCCTAGCAACAGTATTCTCAT pLKO_005 345 CDS 100% 4.950 3.960 N EBAG9 n/a
5 TRCN0000310778 AGCAACATTTGTATGGATTTA pLKO_005 1000 3UTR 100% 13.200 9.240 N EBAG9 n/a
6 TRCN0000303851 AGGACATGACACCAACTATTA pLKO_005 591 CDS 100% 13.200 9.240 N EBAG9 n/a
7 TRCN0000061084 GCCGAACAACAAAGGAAGAAA pLKO.1 860 CDS 100% 5.625 3.938 N EBAG9 n/a
8 TRCN0000299998 GCCGAACAACAAAGGAAGAAA pLKO_005 860 CDS 100% 5.625 3.938 N EBAG9 n/a
9 TRCN0000061086 GCACAGGTTTCTCTAGTAGAT pLKO.1 675 CDS 100% 4.950 3.465 N EBAG9 n/a
10 TRCN0000299999 GCACAGGTTTCTCTAGTAGAT pLKO_005 675 CDS 100% 4.950 3.465 N EBAG9 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004215.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02095 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02095 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000473623 CCGACGAGGGCTCGACGATAGCGA pLX_317 59.1% 99.8% 97.1% V5 (not translated due to prior stop codon) 619delA n/a
4 TRCN0000489314 CTCAAACTTACCCCAGACTTGTGT pLX_317 58.8% 100% 100% V5 (not translated due to prior stop codon) n/a
5 ccsbBroadEn_07369 pDONR223 100% 99.8% 99.5% None 547A>G n/a
6 ccsbBroad304_07369 pLX_304 0% 99.8% 99.5% V5 547A>G n/a
7 TRCN0000473938 AGAGGTTTGCTATCCCGTCCTCCG pLX_317 81.9% 99.8% 99.5% V5 547A>G n/a
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