Construct: ORF TRCN0000473950
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012732.1_s317c1
- Derived from:
- ccsbBroadEn_00134
- DNA Barcode:
- ATAAGACTCTCGACTATAATACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V1B2 (526)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473950
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 526 | ATP6V1B2 | ATPase H+ transporting V1 s... | NM_001693.4 | 100% | 100% | |
| 2 | human | 526 | ATP6V1B2 | ATPase H+ transporting V1 s... | XR_002956632.1 | 17.3% | 1_32del;1110_2553del;3010_8843del | |
| 3 | human | 526 | ATP6V1B2 | ATPase H+ transporting V1 s... | XR_002956633.1 | 16.4% | 1_32del;958_2869del;3478_9311del | |
| 4 | mouse | 11966 | Atp6v1b2 | ATPase, H+ transporting, ly... | NM_007509.3 | 89.4% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1599
- ORF length:
- 1533
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctgcgggcg atgcggggga ttgtcaacgg ggccgcaccc gagctacccg 121 tgcccaccgg tgggccggcg gtgggagctc gggagcaggc gctggcagtc agtcggaact 181 acctctccca gcctcgcctc acatacaaga cagtatctgg agtcaatggt ccactagtga 241 tcttagatca tgttaagttt cccaggtatg ctgaaattgt ccatttgacc ttaccggatg 301 gcacaaagag aagtgggcaa gttctggaag ttagtggttc caaggcagta gttcaggtat 361 ttgaagggac ttcaggtata gatgctaaga aaacgtcctg tgagtttact ggggatattc 421 tccgaacacc ggtgtctgag gatatgcttg gtcgggtatt caatggatcg ggaaaaccca 481 ttgacagagg tcctgttgta ctggccgaag acttccttga tatcatgggt cagccaatca 541 accctcaatg tcgaatctac ccagaggaaa tgattcagac tggcatttcg gccatcgatg 601 ggatgaacag tattgctagg gggcagaaaa ttcctatctt ctctgctgct gggctaccac 661 acaatgagat tgcagctcag atctgtcgcc aggctggttt ggtaaagaaa tccaaagatg 721 tagtagacta cagtgaggaa aattttgcaa ttgtatttgc tgctatgggt gtaaacatgg 781 aaactgcccg gttcttcaaa tctgactttg aagaaaatgg ctcaatggac aatgtctgcc 841 tctttttgaa cttggctaat gacccaacca ttgagcgaat tatcactcct cgcctggctc 901 taaccacagc tgaatttctg gcgtaccaat gtgagaaaca tgtattggtt attctaacag 961 acatgagttc ttatgctgaa gcacttcgag aggtttcagc agccagggaa gaggtacctg 1021 gtcgacgagg ttttccaggt tacatgtata cagatttagc cacgatatat gaacgcgctg 1081 ggcgagtgga agggagaaac ggctcgatta ctcaaatccc tattctaacc atgcctaatg 1141 atgatatcac tcaccccatc ccagacttGA CTGGCTACAT TACAGAGGGG CAGATCTATG 1201 TGGACAGACA GCTGCACAAC AGACAGATTT ATCCACCTAT CAATGTGCTG CCCTCACTAT 1261 CACGGTTAAT GAAGTCTGCT ATTGGAGAAG GGATGACCAG GAAGGATCAT GCCGATGTAT 1321 CTAACCAGCT ATATGCGTGC TATGCTATTG GAAAGGATGT GCAAGCCATG AAAGCTGTCG 1381 TTGGAGAAGA AGCCCTTACC TCAGATGATC TTCTCTACTT GGAATTTCTG CAGAAGTTTG 1441 AGAGGAACTT CATTGCTCAG GGTCCTTACG AAAATCGCAC TGTCTTTGAG ACTTTGGACA 1501 TTGGCTGGCA GCTACTCCGA ATCTTCCCCA AAGAAATGCT GAAGAGAATC CCTCAGAGCA 1561 CCCTCAGCGA ATTTTACCCT CGAGACTCTG CAAAGCATTA CCCAACTTTC TTGTACAAAG 1621 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1681 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1741 ACGAATAAGA CTCTCGACTA TAATACGGAC GCGTTAAGTC gacaatcaac ctctggatta 1801 caaaatttgt gaaagatt