Construct: ORF TRCN0000473965
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004417.1_s317c1
- Derived from:
- ccsbBroadEn_12693
- DNA Barcode:
- TCACACCATTCCACAGGCAGCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEP70 (80321)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473965
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001320600.1 | 99.8% | 99.5% | 404G>A |
| 2 | human | 80321 | CEP70 | centrosomal protein 70 | XM_011513209.3 | 50.3% | 50.2% | 404G>A;637_1260del |
| 3 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001288966.1 | 38.2% | 38% | 404G>A;637_1662del |
| 4 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001320598.2 | 35.4% | 35.3% | 404G>A;637_1791del |
| 5 | human | 80321 | CEP70 | centrosomal protein 70 | NM_024491.4 | 35.4% | 35.3% | 404G>A;637_1791del |
| 6 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007275.1 | 35.4% | 35.3% | 404G>A;637_1791del |
| 7 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001320599.2 | 35.3% | 35.2% | 404G>A;637_1794del |
| 8 | human | 80321 | CEP70 | centrosomal protein 70 | XM_024453781.1 | 35.3% | 35.2% | 404G>A;637_1794del |
| 9 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007271.1 | 33.4% | 33.3% | 404G>A;637_1896del |
| 10 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007272.1 | 33.4% | 33.3% | 404G>A;637_1896del |
| 11 | human | 80321 | CEP70 | centrosomal protein 70 | XM_024453780.1 | 33.4% | 33.3% | 404G>A;637_1896del |
| 12 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001288964.2 | 32.4% | 32.3% | 0_1ins54;350G>A;583_1737del |
| 13 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001288965.2 | 32.1% | 31.9% | (many diffs) |
| 14 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007273.1 | 30.3% | 30.2% | (many diffs) |
| 15 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007274.1 | 30.3% | 30.2% | (many diffs) |
| 16 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007276.2 | 14.8% | 14.7% | 0_1ins354;50G>A;283_1542del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 702
- ORF length:
- 636
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tccggtagcc cctaaacccc aggattccag tcaaccaTCA GACAGACTCA 121 TGACTGAAAA ACAGCAGGAA GAAGCAGAAT GGGAAAGCAT AAATGTGCTA TTGATGATGC 181 ATGGCTTAAA ACCTTTGTCT CTAGTCAAAA GAACAGATCT CAAAGATCTC ATCATTTTTG 241 ACAAACAGTC ATCACAAAGG ATGAGACAGA ATTTGAAATT GTTGGTGGAA GAAACATCAT 301 GTCAACAGAA CATGATACAG GAGCTTATAG AAACTAATCA ACAGCTTAGA AATGAACTTC 361 AGCTAGAGCA AAGCCGAGCA GCCAATCAAG AACAACGAGC TAATGACTTG GAACAAATTA 421 TGGAAAGTGT GAAATCCAAA ATTGGTGAAT TGGAGGATGA ATCACTAAAT AGGGCTTGCC 481 ACCAACAGAA TAAAATAAAA GATCTTCAAA AGGAGCAGAA AACTTTACAG GTGAAGTGCC 541 AGCATTATAA GAAAAAACGA ACGGAGCAAG AAGAAACTAT TGCTTCTTTG CAAATGGAAG 601 TCTGTAGATT AAAAAAGGAG GAAGAAGATC GCATTGTCAC TCAAAACAGA GTGTTTGCCT 661 ATCTGTGCAA AAGAGTTCCT CATACCGTCT TGGATAGACA GTACCCAACT TTCTTGTACA 721 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 781 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 841 AGGACGATCA CACCATTCCA CAGGCAGCGC AACGCGTTAA GTCgacaatc aacctctgga 901 ttacaaaatt tgtgaaagat t