Construct: ORF TRCN0000473967
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018007.1_s317c1
- Derived from:
- ccsbBroadEn_15887
- DNA Barcode:
- CGATAAACCCCATATGAGTACGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRRC8D (55144)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473967
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55144 | LRRC8D | leucine rich repeat contain... | NM_001134479.2 | 16.5% | 16% | (many diffs) |
| 2 | human | 55144 | LRRC8D | leucine rich repeat contain... | NM_018103.5 | 16.5% | 16% | (many diffs) |
| 3 | human | 55144 | LRRC8D | leucine rich repeat contain... | XM_011541689.1 | 16.5% | 16% | (many diffs) |
| 4 | human | 55144 | LRRC8D | leucine rich repeat contain... | XM_017001601.2 | 16.5% | 16% | (many diffs) |
| 5 | human | 55144 | LRRC8D | leucine rich repeat contain... | XM_017001602.1 | 16.5% | 16% | (many diffs) |
| 6 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | NM_001122768.1 | 14.1% | 16% | (many diffs) |
| 7 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | NM_178701.3 | 14.1% | 16% | (many diffs) |
| 8 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | XM_006534893.2 | 14.1% | 16% | (many diffs) |
| 9 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | XM_006534894.3 | 14.1% | 16% | (many diffs) |
| 10 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | XM_006534895.3 | 14.1% | 16% | (many diffs) |
| 11 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | XM_006534896.3 | 14.1% | 16% | (many diffs) |
| 12 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | XM_011249436.1 | 14.1% | 16% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 519
- ORF length:
- 429
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGTCAGTAG CAACTTTTGG TTCAAATATC 121 CCAAAACATG CTCAAAAGTA GAACATTTTG TTTCAATATT AGGAAAGTGC TTTGAATCCC 181 CTTGGACGAC AAAAGCGTTG TCTGAGACAG CATGCGAAGA CTCAGAGGAA AACAAGCAGA 241 GAATAACAGG TGCCCAGACT CTACCAAAGC ATGTTTCTAC CAGCAGTGAT GAAGGGAGCC 301 CCAGTGCCAG TACACCAATG ATCAATAAAA CTGGCTTTAA ATTTTCAGCT GAGAAGCCTG 361 TGATTGAAGT TCCCAGCATG ACAATCCTGG ATAAAAAGGA TGGAGAGCAG GCCAAAGCCC 421 TGTTTGAGAA AGTGAGGAAG TTCCGTGCCC ATGTGGAAGA TAGTGACTTG ATCTATAAAC 481 TCTATGTGGT CCAAACAGCT TCTCCCTTTC CCAACCAATA CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGACGATAA ACCCCATATG AGTACGCCAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt