Construct: ORF TRCN0000473971
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016914.1_s317c1
- Derived from:
- ccsbBroadEn_08038
- DNA Barcode:
- TTACTTCAGTTAATACATAATTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- HBP1 (26959)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473971
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26959 | HBP1 | HMG-box transcription factor 1 | NM_012257.4 | 99.8% | 94.3% | 534C>T;1456G>T |
| 2 | human | 26959 | HBP1 | HMG-box transcription factor 1 | XM_005250266.3 | 99.8% | 94.3% | 534C>T;1456G>T |
| 3 | human | 26959 | HBP1 | HMG-box transcription factor 1 | XM_005250267.3 | 99.8% | 94.3% | 534C>T;1456G>T |
| 4 | human | 26959 | HBP1 | HMG-box transcription factor 1 | XM_017011967.2 | 99.8% | 94.3% | 534C>T;1456G>T |
| 5 | human | 26959 | HBP1 | HMG-box transcription factor 1 | XM_024446713.1 | 99.8% | 94.3% | 534C>T;1456G>T |
| 6 | human | 26959 | HBP1 | HMG-box transcription factor 1 | XM_024446714.1 | 99.8% | 94.3% | 534C>T;1456G>T |
| 7 | human | 26959 | HBP1 | HMG-box transcription factor 1 | NM_001244262.1 | 97.9% | 92.5% | 1_30del;564C>T;1486G>T |
| 8 | human | 26959 | HBP1 | HMG-box transcription factor 1 | XR_002956419.1 | 77.9% | (many diffs) | |
| 9 | human | 26959 | HBP1 | HMG-box transcription factor 1 | XR_001744628.1 | 51.6% | (many diffs) | |
| 10 | human | 26959 | HBP1 | HMG-box transcription factor 1 | XR_002956420.1 | 44.2% | (many diffs) | |
| 11 | mouse | 73389 | Hbp1 | high mobility group box tra... | XM_017315212.1 | 91.9% | 89.1% | (many diffs) |
| 12 | mouse | 73389 | Hbp1 | high mobility group box tra... | NM_153198.2 | 90.1% | 87.4% | (many diffs) |
| 13 | mouse | 73389 | Hbp1 | high mobility group box tra... | XM_006515228.3 | 84.4% | 82.1% | (many diffs) |
| 14 | mouse | 73389 | Hbp1 | high mobility group box tra... | XM_006515229.3 | 84.4% | 82.1% | (many diffs) |
| 15 | mouse | 73389 | Hbp1 | high mobility group box tra... | XM_006515227.3 | 82.9% | 80.7% | (many diffs) |
| 16 | mouse | 73389 | Hbp1 | high mobility group box tra... | NM_177993.3 | 80.9% | 87.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1521
- ORF length:
- 1455
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gtgggaagtg aagacaaatc agatgcctaa tgcagtacag aaactcctgt 121 tggtgatgga caagagagcc tcaggaatga atgactcatt ggagttgctg cagtgtaatg 181 agaatttgcc atcttcacct ggatataact cctgtgatga acacatggag cttgatgacc 241 ttcctgaact tcaggcagtt caaagtgatc ctacccaatc tggcatgtac cagctgagtt 301 cagatgtttc acatcaagaa tacccaagat catcttggaa ccaaaatacc tcagacatac 361 cagaaactac ttaccgtgaa aatgaggtgg actggctaac agaattggca aatatcgcga 421 ccagtccaca aagtccactg atgcagtgct cattttacaa tagatcatct cctgtacaca 481 tcatagccac tagcaaaagt ttacattcct atgcacgccc tccaccagtg tcctcttctt 541 cgaagagtga accagccttc cctcatcacc attggaagga ggaaacacca gtaagacatg 601 aaagggcaaa tagtgagtca gaatctggca ttttctgcat gtcctccctg tcagatgatg 661 atgatttggg atggtgcaat tcctggcctt caactgtctg gcactgtttt ttgaaaggca 721 cacgactgtg ctttcataag ggaagcaata aggaatggca agatgttgaa gattttgcta 781 gagctgaagg ctgtgataat gaggaagatc ttcaaatggg cattcacaag ggctatggtt 841 ctgatggtct aaagttgtta tcacatgaag aaagtgtatc atttggcgag tctgtactga 901 agttgacttt tgatcctggt acagtagaag atggtttact taccgtagag tgtaagctgg 961 accacccttt ctatgttaaa aataaaggtt ggtcatcatt ttatccaagc ttgactgtgg 1021 tacagcatgg cattccatgt tgtgaagttc atattggcga tgtatgtcta ccTCCTGGAC 1081 ACCCCGATGC CATTAATTTT GATGATTCAG GTGTTTTTGA TACATTTAAA AGCTATGACT 1141 TCACACCTAT GGATTCTTCT GCAGTTTATG TGTTAAGTAG TATGGCTCGC CAGCGTCGTG 1201 CATCTTTGTC TTGTGGAGGA CCTGGTGGTC AAGACTTTGC AAGATCTGGA TTCAGTAAAA 1261 ACTGTGGCTC ACCTGGATCA TCACAGCTCT CTTCCAATTC TTTGTATGCT AAAGCTGTCA 1321 AAAACCACAG CTCAGGGACT GTGAGTGCCA CTTCTCCTAA TAAGTGCAAA AGACCAATGA 1381 ATGCCTTCAT GCTTTTTGCC AAAAAATACA GAGTTGAATA TACTCAGATG TATCCAGGGA 1441 AAGATAACAG AGCCATAAGT GTGATCCTTG GTGACAGGTG GAAGAAAATG AAGAATGAAG 1501 AGAGAAGAAT GTACACATTA TAAGCAAAGG CTTTGGCTGA AGAACAGAAA CGTTTAAATC 1561 CTGACTGTTG GAAGAGGAAA AGAACCAATT CAGGCTCACA ACAACATTAC CCAACTTTCT 1621 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1681 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1741 GTGGAAAGGA CGATTACTTC AGTTAATACA TAATTTTACG CGTTAAGTCg acaatcaacc 1801 tctggattac aaaatttgtg aaagatt