Construct: ORF TRCN0000473988
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015643.1_s317c1
- Derived from:
- ccsbBroadEn_12534
- DNA Barcode:
- CTATGCCCTACGCCATCGACTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX54 (79039)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473988
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79039 | DDX54 | DEAD-box helicase 54 | NM_001111322.2 | 23.5% | 21.5% | (many diffs) |
| 2 | human | 79039 | DDX54 | DEAD-box helicase 54 | NM_024072.4 | 23.4% | 21.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 738
- ORF length:
- 672
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cctcggatgt ggagccggac acccgggaga tggtgcgtgc ccagaacaag 121 aagaagaaga agtctggagg cttccagtcc atgggcctga gctacccggt gttcaaaggc 181 atcatgaaga agggggagcc tttgagcagc aggcagctgg cgctgtcctg gacttgatgg 241 gggatgaagc ccagaacctg acgaggggcc GGCAGCAGCT CAAGTGGGAC CGTAAGAAGA 301 AGCGGTTTGT GGGACAGTCA GGACAGGAAG ACAAGAAGAA GATTAAGACA GAGAGCGGCC 361 GCTACATCAG CAGCTCCTAC AAGCGAGACC TCTATCAGAA GTGGAAACAG AAACAGAAAA 421 TTGATGATCG TGACTCGGAC GAAGAAGGGG CATCTGACCG GCGAGGCCCA GAGCGAAGAG 481 GTGGGAAGCG AGACCGTGGC CAAGCAGGTG CATCCCGGCC CCACGCCCCA GGCACCCCTG 541 CAGGCCGAGT CCGCCCGGAA CTCAAGACCA AGCAGCAGAT CCTGAAGCAG CGGCGCCGGG 601 CCCAGAAGCT GCACTTCCTG CAGCGTGGTG GCCTCAAGCA GCTCTCTGCC CGCAACCGCC 661 GCCGCGTCCA GGAGCTGCAG CAGGGCGCCT TCGGCCGGGG TGCCCGCTCC AAGAAGGGCA 721 AGATGCGGAA GAGGATGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 781 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 841 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTATGCC CTACGCCATC 901 GACTTTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt