Construct: ORF TRCN0000474008
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017352.1_s317c1
- Derived from:
- ccsbBroadEn_03388
- DNA Barcode:
- TAATCGAAAAGCGATACAGCGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZFYVE1 (53349)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474008
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53349 | ZFYVE1 | zinc finger FYVE-type conta... | NM_021260.4 | 100% | 100% | |
| 2 | human | 53349 | ZFYVE1 | zinc finger FYVE-type conta... | NM_001281734.2 | 98.1% | 98.1% | 1516_1517ins42 |
| 3 | human | 53349 | ZFYVE1 | zinc finger FYVE-type conta... | XR_001750376.2 | 50.6% | 1_645del;2063_2064ins98;2879_4312del | |
| 4 | human | 53349 | ZFYVE1 | zinc finger FYVE-type conta... | NM_001281735.1 | 46.5% | 46.5% | 0_1ins1245 |
| 5 | human | 53349 | ZFYVE1 | zinc finger FYVE-type conta... | NM_178441.2 | 46.5% | 46.5% | 0_1ins1245 |
| 6 | human | 53349 | ZFYVE1 | zinc finger FYVE-type conta... | XM_017021373.1 | 46.5% | 46.5% | 0_1ins1245 |
| 7 | mouse | 217695 | Zfyve1 | zinc finger, FYVE domain co... | NM_183154.3 | 90.1% | 96.6% | (many diffs) |
| 8 | mouse | 217695 | Zfyve1 | zinc finger, FYVE domain co... | XM_006515689.3 | 90.1% | 96.6% | (many diffs) |
| 9 | mouse | 217695 | Zfyve1 | zinc finger, FYVE domain co... | XM_006515690.3 | 90.1% | 96.6% | (many diffs) |
| 10 | mouse | 217695 | Zfyve1 | zinc finger, FYVE domain co... | XM_006515691.2 | 90.1% | 96.6% | (many diffs) |
| 11 | mouse | 217695 | Zfyve1 | zinc finger, FYVE domain co... | XM_006515692.3 | 41.9% | 45.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2397
- ORF length:
- 2331
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgcccagact tccccagcag agaagggcct gaatccgggg ctgatgtgcc 121 aggaaagtta cgcttgcagc gggactgatg aagctatctt tgagtgtgat gagtgctgca 181 gtctgcagtg tctccgctgc gaggaggagc tccatcggca ggagcgcctg agaaaccatg 241 agcggataag actcaaacct ggccatgtcc cttactgtga cctctgcaag ggtctcagtg 301 ggcatttacc aggtgttagg cagagggcaa tagtgaggtg ccagacctgc aaaattaact 361 tgtgcctgga gtgccagaag aggactcatt ctgggggtaa caaaaggaga caccctgtta 421 ctgtgtacaa tgtcagtaat ctccaggagt cactggaggc agaagagatg gatgaggaga 481 ccaagaggaa gaagatgact gagaaggttg tgagtttcct cctagtagac gaaaatgaag 541 aaattcaggt aacaaatgaa gaagacttta ttagaaaatt ggactgcaaa cctgatcagc 601 atctgaaagt ggtttccatt tttggaaata ctggtgatgg aaagtctcat actctcaacc 661 acactttctt ttatggtcgt gaagtcttta aaacctcccc gacccaggag tcctgcactg 721 tgggagtgtg ggcagcctat gacccagttc acaaagtagc agtgatcgat acggaagggc 781 tcctgggggc caccgtgaat ctaagccaga gaacacggct gctgcttaag gtcctggcca 841 tctcagacct cgtcatctat cgaactcatg cagaccggct gcataacgac ctcttcaaat 901 tccttgggga tgcctcagaa gcttatctga agcacttcac caaggagctc aaggccacca 961 ctgctcgctg tggcctggat gtccctttat ccacactggg ccctgcagtt atcatcttcc 1021 atgagaccgt gcacacccag ctactgggct ctgatcatcc ctcagaggtg ccagagaagc 1081 tcatccagga ccggttccgg aagctgggcc gtttccctga agcctttagt tccattcact 1141 acaagggaac gaggacttac aaccctccca cggacttttc tgggcttcgg cgtgctttgg 1201 agcagctact agagaataac accacccgtt ctccccggca cccgggagtc atcttcaaag 1261 ccctgaaggc actaagtgac cgcttcagcg gtgagatccc cgatgaccag atggcgcaca 1321 gctccttttt tccagatgag tatttcacct gctcctcctt gtgcctcagc tgtggggttg 1381 gatgtaagaa aagcatgaat catgggaagg aaggagtgcc tcatgaagcc aagagccgct 1441 gcagatactc ccaccagtat gacaaccgag tgtatacctg caaggcctgc tatgagagag 1501 gcgaggaagt cagtgtagtg cccaaaacat ctgcttccac tgactccccc tggatgggtc 1561 tcgcaaaata tgcctggtct gggtatgtga tcgaatgtcc taactgtggc gtggtctatc 1621 gtagtcggca gtactggttt ggaaaccaag atcctgtgga tacggtggtg cggacagaga 1681 ttgtgcatgt gtggcctgga actgatgggt ttctgaagga caacaacaat gctgcccagc 1741 gcctgttgga cgggatgaac ttcatggctc agtcggtgtc cgagcttagc cttggaccca 1801 ccaaggctgt gacttcctgg ctgacagacc agatcgcccc tgcctactgg aggcccaact 1861 cccagattct gagctgcaac aagtgtgcga cgtcctttaa agataacgac actaagcatc 1921 actgccgagc ctgtggggag ggcttctgtg acagctgttc atcaaagact cggccagtgc 1981 ctgagcgggg ctggggccct gcgccagtgc gggtctgtga caactgcTAC GAAGCCAGGA 2041 ACGTCCAGTT AGCTGTTACC GAGGCACAAG TGGACGATGA AGGTGGAACG CTCATTGCTC 2101 GGAAGGTGGG CGAGGCCGTG CAGAACACTC TGGGAGCCGT GGTGACAGCC ATTGACATAC 2161 CACTAGGTCT GGTAAAGGAC GCGGCCAGGC CTGCGTACTG GGTGCCTGAC CACGAAATCC 2221 TCCACTGCCA CAACTGCCGG AAGGAGTTCA GCATCAAGCT CTCCAAGCAC CACTGCCGGG 2281 CCTGCGGACA GGGCTTCTGT GATGAGTGCT CCCATGACCG CCGGGCTGTT CCTTCTCGTG 2341 GCTGGGACCA TCCCGTCCGA GTCTGCTTCA ACTGCAATAA AAAGCCCGGT GACCTTTACC 2401 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2461 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2521 ATATATCTTG TGGAAAGGAC GATAATCGAA AAGCGATACA GCGACGACGC GTTAAGTCga 2581 caatcaacct ctggattaca aaatttgtga aagatt