Construct: ORF TRCN0000474017
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017004.1_s317c1
- Derived from:
- ccsbBroadEn_01163
- DNA Barcode:
- GATACTATTAATTACCGACAGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDHA1 (5160)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474017
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5160 | PDHA1 | pyruvate dehydrogenase E1 a... | NM_000284.4 | 100% | 100% | |
| 2 | human | 5160 | PDHA1 | pyruvate dehydrogenase E1 a... | NM_001173455.1 | 98.2% | 98.2% | 289_309del |
| 3 | human | 5160 | PDHA1 | pyruvate dehydrogenase E1 a... | NM_001173456.1 | 92% | 92% | 507_508ins93 |
| 4 | human | 5160 | PDHA1 | pyruvate dehydrogenase E1 a... | NM_001173454.1 | 91.1% | 91.1% | 57_170del |
| 5 | human | 5160 | PDHA1 | pyruvate dehydrogenase E1 a... | XM_017029574.2 | 83.8% | 83.8% | 57_170del;621_622ins93 |
| 6 | human | 5161 | PDHA2 | pyruvate dehydrogenase E1 a... | NM_005390.5 | 83.1% | 85.1% | (many diffs) |
| 7 | mouse | 18597 | Pdha1 | pyruvate dehydrogenase E1 a... | NM_008810.3 | 88.5% | 98.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gaagatgctc gccgccgtct cccgcgtgct gtctggcgct tctcagaagc 121 cggcaagcag agtgctggta gcatcccgta attttgcaaa tgatgctaca tttgaaatta 181 agaaatgtga ccttcaccgg ctggaagaag gccctcctgt cacaacagtg ctcaccaggg 241 aggatgggct caaatactac aggatgatgc agactgtacg ccgaatggag ttgaaagcag 301 atcagctgta taaacagaaa attattcgtg gtttctgtca cttgtgtgat ggtcaggaag 361 cttgctgtgt gggcctggag gccggcatca accccacaga ccatctcatc acagcctacc 421 gggctcacgg ctttactttc acccggggcc tttccgtccg agaaattctc gcagagctta 481 caggacgaaa aggaggttgt gctaaaggga aaggaggatc gatgcacatg tatgccaaga 541 acttctacgg gggcaatggc atcgtgggag cgcaggtgcc cctgggcgct gggattgctc 601 tagcctgtaa gtataatgga aaagatgagg tctgcctgac tttatatggc gatggtgctg 661 ctaaccaggg ccagatattc gaagcttaca acatggcagc tttgtggaaa ttacctTGTA 721 TTTTCATCTG TGAGAATAAT CGCTATGGAA TGGGAACGTC TGTTGAGAGA GCGGCAGCCA 781 GCACTGATTA CTACAAGAGA GGCGATTTCA TTCCTGGGCT GAGAGTGGAT GGAATGGATA 841 TCCTGTGCGT CCGAGAGGCA ACAAGGTTTG CTGCTGCCTA TTGTAGATCT GGGAAGGGGC 901 CCATCCTGAT GGAGCTGCAG ACTTACCGTT ACCACGGACA CAGTATGAGT GACCCTGGAG 961 TCAGTTACCG TACACGAGAA GAAATTCAGG AAGTAAGAAG TAAGAGTGAC CCTATTATGC 1021 TTCTCAAGGA CAGGATGGTG AACAGCAATC TTGCCAGTGT GGAAGAACTA AAGGAAATTG 1081 ATGTGGAAGT GAGGAAGGAG ATTGAGGATG CTGCCCAGTT TGCCACGGCC GATCCTGAGC 1141 CACCTTTGGA AGAGCTGGGC TACCACATCT ACTCCAGCGA CCCACCTTTT GAAGTTCGTG 1201 GTGCCAATCA GTGGATCAAG TTTAAGTCAG TCAGTTACCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 AGATACTATT AATTACCGAC AGACCACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt