Construct: ORF TRCN0000474019
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000404.1_s317c1
- Derived from:
- ccsbBroadEn_06953
- DNA Barcode:
- TCGCTGATGCGTAGGCGGACCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLA (6503)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474019
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6503 | SLA | Src like adaptor | NM_001045556.3 | 99.8% | 100% | 324C>T |
| 2 | human | 6503 | SLA | Src like adaptor | NM_001045557.3 | 94% | 94.1% | 1_51del;375C>T |
| 3 | human | 6503 | SLA | Src like adaptor | NM_006748.4 | 87.2% | 87.3% | 1_120del;444C>T |
| 4 | human | 6503 | SLA | Src like adaptor | NM_001282964.2 | 79% | 79.1% | 1_51del;375C>T;402_403ins132 |
| 5 | human | 6503 | SLA | Src like adaptor | NM_001282965.2 | 60.8% | 60.8% | 0_1ins324 |
| 6 | human | 6503 | SLA | Src like adaptor | XM_017013739.2 | 60.8% | 60.8% | 0_1ins324 |
| 7 | mouse | 20491 | Sla | src-like adaptor | NM_009192.3 | 80.3% | 86.4% | (many diffs) |
| 8 | mouse | 20491 | Sla | src-like adaptor | XM_011245530.2 | 80.3% | 86.4% | (many diffs) |
| 9 | mouse | 20491 | Sla | src-like adaptor | NM_001029841.4 | 76% | 81.8% | (many diffs) |
| 10 | mouse | 20491 | Sla | src-like adaptor | XM_017316522.1 | 70.6% | 75.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 894
- ORF length:
- 828
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aaacagcatg aaatccaccc ctgcgcctgc cgagaggccc ctgcccaacc 121 cggagggact ggatagcgac ttccttgccg tgctaagtga ctacccgtct cctgacatca 181 gccccccgat attccgccga ggggagaaac tgcgtgtgat ttctgatgaa gggggctggt 241 ggaaagctat ttctcttagc actggtcgag agagttacat ccctggaata tgtgtggcca 301 gagtttacca tggctggctg tttgagggcc tgggcagaga caaggccgag gagctgctgc 361 agctgccaga cacaaaggtc ggctccttta tgatcagaga gagtgagacc aagaaagggt 421 tttactcact gtcggtgaga cacaggcagg taaagcatta ccgcattttc cgtctgccca 481 acaactggta ctacatttcc ccgaggctca ccTTCCAGTG CCTGGAGGAC CTGGTGAACC 541 ACTATTCTGA GGTGGCTGAT GGCCTGTGCT GTGTGCTCAC CACGCCCTGC CTGACACAAA 601 GCACGGCTGC CCCAGCAGTG AGGGCCTCCA GCTCACCTGT CACCTTGCGT CAGAAGACTG 661 TGGACTGGAG GAGAGTGTCC AGACTGCAGG AGGACCCCGA GGGAACAGAG AACCCGCTTG 721 GGGTAGACGA GTCCCTTTTC AGCTATGGCC TTCGAGAGAG CATTGCCTCT TACCTGTCCC 781 TGACCAGTGA GGACAACACC TCCTTTGATC GAAAGAAGAA AAGCATCTCC CTGATGTATG 841 GTGGCAGCAA GAGAAAGAGC TCATTCTTCT CATCACCACC TTACTTTGAG GACTACCCAA 901 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 961 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1021 TATCTTGTGG AAAGGACGAT CGCTGATGCG TAGGCGGACC CCCACGCGTT AAGTCgacaa 1081 tcaacctctg gattacaaaa tttgtgaaag att