Construct: ORF TRCN0000474028
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009233.1_s317c1
- Derived from:
- ccsbBroadEn_00145
- DNA Barcode:
- TACACCTATATAATTACTGTGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BACH1 (571)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474028
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 571 | BACH1 | BTB domain and CNC homolog 1 | NM_001186.4 | 100% | 100% | |
| 2 | human | 571 | BACH1 | BTB domain and CNC homolog 1 | NM_206866.3 | 100% | 100% | |
| 3 | human | 571 | BACH1 | BTB domain and CNC homolog 1 | NR_027655.2 | 72.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2274
- ORF length:
- 2208
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tctgagtgag aactcggttt ttgcctatga atcttctgtg catagcacca 121 atgttttact cagccttaat gaccagcgga agaaagatgt gctgtgcgat gtcaccatct 181 ttgtggaggg acagcggttc cgcgctcacc ggtccgtgct ggcggcatgc agcagttact 241 tccactcaag aatcgtaggc caggctgatg gagagctgaa cattactctt ccagaagagg 301 tgacagttaa aggatttgaa cctttaattc agtttgccta cactgctaaa ctgattttaa 361 gtaaagagaa tgtggatgaa gtgtgcaaat gtgtggagtt tttaagtgta cataatattg 421 aggaatcctg ctttcagttt ctgaaattta agtttttgga ctccactgca gaccagcaag 481 aatgcccaag aaaaaaatgc ttttcatcac actgtcagaa aacagacctt aaactttcac 541 ttttggacca gagggatcta gaaactgatg aagtggagga atttctggaa aataaaaatg 601 ttcagactcc tcagtgtaaa ctccgcaggt atcaaggaaa tgcaaaagcc tcacctcctc 661 tacaagacag tgccagtcag acatatgagt ccatgtgctt agagaaggat gctgctctgg 721 ccttgccttc tttatgcccc aaatacagaa aattccaaaa agcatttgga actgacagag 781 tccgtactgg ggaatctagt gtcaaagaca ttcatgcttc tgttcagcca aatgaaaggt 841 ctgaaaatga atgcctggga ggagtcccgg agtgtagaga tttgcaggtg atgttaaaat 901 gtgacgaaag taaattagca atggaacctg aagaaacgaa gaaagatcct gcttctcagt 961 gcccaactga aaaatcagaa gtgactcctt tcccccacaa ttcttccata gaccctcatg 1021 gactttattc tttgtctctt ttacacacat atgaccaata tggtgacttg aattttgctg 1081 gtatgcaaaa cacaacagtg ttaacagaaa agcctttgtc aggtacagac gtccaagaaa 1141 aaacatttgg tgaaagtcag gatttacctt tgaaatccga cttgggcacc agggaagata 1201 gtagtgttgc atctagtgat aggagtagtg tggagcgaga agtggcagaa cacctagcaa 1261 aaggcttctg gagtgacatt tgcagcacgg acactccttg ccaaatgcag ttatcacctg 1321 ctgtggccaa agatggctca gaacagatct cacagaaacg gtctgagtgt ccgtggttag 1381 gtatcaggat tagtgagagc ccagaaccag gtcaaaggac tttcacaaca ttaagttctg 1441 tcaactgccc ttttataagt actctgagta ctgaaggctg ttcaagcaat ttggaaattg 1501 gaaacgatga ttatgtttca gaaccccagc aagaaccttg cccatatgct tgtgtcatta 1561 gcttgggaga cgactctgag acggacaccg aaggagacag tgaatcctgt tcagccagag 1621 aacaagaatg tgaggtaaaa ctgccattca atgcacaacg gataatttca ctgtctcgaa 1681 atgattttca gtccttgttg aaaatgcaca agcttactcc agaacagctg gattgtatCC 1741 ATGATATTCG AAGAAGAAGT AAAAACAGAA TTGCTGCACA GCGCTGTCGC AAGAGAAAAC 1801 TTGACTGTAT ACAGAATCTT GAATCAGAAA TTGAGAAGCT GCAAAGTGAA AAGGAGAGCT 1861 TGTTGAAGGA AAGAGATCAC ATTTTGTCAA CTCTGGGTGA GACAAAGCAG AACCTAACTG 1921 GACTTTGCCA GAAAGTTTGT AAAGAAGCAG CTCTGAGTCA AGAACAAATA CAGATACTCG 1981 CCAAGTACTC AGCTGCAGAT TGCCCACTTT CATTTTTAAT TTCTGAAAAA GATAAAAGTA 2041 CTCCTGATGG TGAACTGGCG TTACCATCAA TTTTCAGTTT ATCTGACCGG CCTCCAGCAG 2101 TGCTGCCTCC CTGTGCCAGA GGAAACAGTG AGCCTGGCTA CGCGCGAGGG CAGGAGTCCC 2161 AGCAGATGTC CACAGCCACC TCTGAGCAAG CTGGGCCTGC GGAACAGTGT CGTCAGAGTG 2221 GTGGGATCTC AGATTTCTGT CAGCAGATGA CTGATAAATG TACTACTGAT GAGTACCCAA 2281 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2341 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2401 TATCTTGTGG AAAGGACGAT ACACCTATAT AATTACTGTG AGTACGCGTT AAGTCgacaa 2461 tcaacctctg gattacaaaa tttgtgaaag att