Construct: ORF TRCN0000474035
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018415.1_s317c1
- Derived from:
- ccsbBroadEn_00883
- DNA Barcode:
- AGGCTTCCTAATGATCTTCTGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ISG20 (3669)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474035
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_001303233.2 | 99.8% | 100% | 414A>G |
| 2 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_001303234.2 | 99.8% | 100% | 414A>G |
| 3 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_002201.6 | 99.8% | 100% | 414A>G |
| 4 | human | 3669 | ISG20 | interferon stimulated exonu... | XM_005254899.2 | 99.8% | 100% | 414A>G |
| 5 | human | 3669 | ISG20 | interferon stimulated exonu... | XM_006720488.3 | 99.8% | 100% | 414A>G |
| 6 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_001303237.2 | 88.7% | 88.9% | 226_227ins60;354A>G |
| 7 | human | 3669 | ISG20 | interferon stimulated exonu... | XM_017022148.1 | 88.7% | 88.9% | 226_227ins60;354A>G |
| 8 | human | 3669 | ISG20 | interferon stimulated exonu... | XM_011521521.2 | 71.8% | 55.2% | (many diffs) |
| 9 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_001303235.2 | 71.6% | 61.4% | (many diffs) |
| 10 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_001303236.2 | 71.6% | 61.4% | (many diffs) |
| 11 | human | 3669 | ISG20 | interferon stimulated exonu... | XM_017022147.1 | 71.6% | 61.4% | (many diffs) |
| 12 | human | 3669 | ISG20 | interferon stimulated exonu... | NR_130134.2 | 23.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgggagccgt gaggtggtgg ccatggactg cgagatggtg gggctggggc 121 cccaccggga gagtggcctg gctcgttgca gccTCGTGAA CGTCCACGGT GCTGTGCTGT 181 ACGACAAGTT CATCCGGCCT GAGGGAGAGA TCACCGATTA CAGAACCCGG GTCAGCGGGG 241 TCACCCCTCA GCACATGGTG GGGGCCACAC CATTTGCCGT GGCCAGGCTA GAGATCCTGC 301 AGCTCCTGAA AGGCAAGCTG GTGGTGGGTC ATGACCTGAA GCACGACTTC CAGGCACTGA 361 AAGAGGACAT GAGCGGCTAC ACAATCTACG ACACGTCCAC TGACAGGCTG TTGTGGCGTG 421 AGGCCAAGCT GGACCACTGC AGGCGTGTCT CCCTGCGGGT GCTGAGTGAG CGCCTCCTGC 481 ACAAGAGCAT CCAGAACAGC CTGCTTGGAC ACAGCTCGGT GGAAGATGCG AGGGCAACGA 541 TGGAGCTCTA TCAAATCTCC CAGAGAATCC GAGCCCGCCG AGGGCTGCCC CGCCTGGCTG 601 TGTCAGACTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGGCTT CCTAATGATC TTCTGGTAAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt