Construct: ORF TRCN0000474041
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007538.1_s317c1
- Derived from:
- ccsbBroadEn_12096
- DNA Barcode:
- TACTATTTGCTAATCACCCATCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APTX (54840)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474041
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54840 | APTX | aprataxin | NM_001369002.1 | 66.1% | 66.1% | 1_258del |
| 2 | human | 54840 | APTX | aprataxin | NM_001369003.1 | 66.1% | 66.1% | 1_258del |
| 3 | human | 54840 | APTX | aprataxin | NM_001369004.1 | 66.1% | 66.1% | 1_258del |
| 4 | human | 54840 | APTX | aprataxin | NM_001369005.1 | 66.1% | 66.1% | 1_258del |
| 5 | human | 54840 | APTX | aprataxin | NM_001370669.1 | 66.1% | 66.1% | 1_258del |
| 6 | human | 54840 | APTX | aprataxin | NM_001370670.1 | 66.1% | 66.1% | 1_258del |
| 7 | human | 54840 | APTX | aprataxin | NM_001370673.1 | 66.1% | 66.1% | 1_258del |
| 8 | human | 54840 | APTX | aprataxin | XM_011517938.2 | 66.1% | 66.1% | 1_258del |
| 9 | human | 54840 | APTX | aprataxin | NM_001195252.2 | 62.2% | 62.2% | 1_306del |
| 10 | human | 54840 | APTX | aprataxin | NM_001195250.2 | 58.3% | 58.3% | 1_360del |
| 11 | human | 54840 | APTX | aprataxin | NM_001195254.1 | 58.3% | 58.3% | 1_360del |
| 12 | human | 54840 | APTX | aprataxin | NM_001369000.1 | 58.3% | 58.3% | 1_360del |
| 13 | human | 54840 | APTX | aprataxin | NM_001369001.1 | 58.3% | 58.3% | 1_360del |
| 14 | human | 54840 | APTX | aprataxin | NM_001195248.2 | 49.1% | 49.1% | 1_522del |
| 15 | human | 54840 | APTX | aprataxin | NM_001195249.1 | 49.1% | 49.1% | 1_522del |
| 16 | human | 54840 | APTX | aprataxin | NM_001368995.1 | 49.1% | 49.1% | 1_522del |
| 17 | human | 54840 | APTX | aprataxin | NM_001368996.1 | 49.1% | 49.1% | 1_522del |
| 18 | human | 54840 | APTX | aprataxin | NM_001368997.1 | 49.1% | 49.1% | 1_522del |
| 19 | human | 54840 | APTX | aprataxin | NM_001368998.1 | 49.1% | 49.1% | 1_522del |
| 20 | human | 54840 | APTX | aprataxin | NM_175073.2 | 49.1% | 49.1% | 1_522del |
| 21 | human | 54840 | APTX | aprataxin | NM_001369006.1 | 46.4% | 46% | 1_258del;609_610insGCTGTGATC;612_613ins141 |
| 22 | human | 54840 | APTX | aprataxin | NM_001195251.1 | 34.5% | 34.2% | 1_522del;873_874insGCTGTGATC;876_877ins141 |
| 23 | human | 54840 | APTX | aprataxin | NM_001368999.1 | 34.5% | 34.2% | 1_522del;873_874insGCTGTGATC;876_877ins141 |
| 24 | human | 54840 | APTX | aprataxin | NM_175069.3 | 34.5% | 34.2% | 1_522del;873_874insGCTGTGATC;876_877ins141 |
| 25 | human | 54840 | APTX | aprataxin | XM_017014832.1 | 34.2% | 22.9% | (many diffs) |
| 26 | human | 54840 | APTX | aprataxin | XM_017014833.2 | 32.5% | 25.8% | (many diffs) |
| 27 | human | 54840 | APTX | aprataxin | XM_017014831.1 | 31.9% | 22.5% | (many diffs) |
| 28 | human | 54840 | APTX | aprataxin | XM_024447578.1 | 30.3% | 23.1% | (many diffs) |
| 29 | human | 54840 | APTX | aprataxin | XM_017014836.2 | 29.3% | 23.6% | (many diffs) |
| 30 | human | 54840 | APTX | aprataxin | NR_160930.1 | 27.1% | 1_468del;973_1857del | |
| 31 | human | 54840 | APTX | aprataxin | NR_160921.1 | 26.7% | 1_492del;997_1881del | |
| 32 | human | 54840 | APTX | aprataxin | NR_036577.1 | 26.5% | 1_486del;991_1895del | |
| 33 | human | 54840 | APTX | aprataxin | NR_160926.1 | 26.4% | 1_518del;1023_1907del | |
| 34 | human | 54840 | APTX | aprataxin | NR_160923.1 | 26.3% | 1_527del;1032_1916del | |
| 35 | human | 54840 | APTX | aprataxin | NR_160924.1 | 26.2% | 1_532del;1037_1921del | |
| 36 | human | 54840 | APTX | aprataxin | NR_160931.1 | 24% | 1_707del;1212_2096del | |
| 37 | human | 54840 | APTX | aprataxin | NR_160922.1 | 23.8% | 1_723del;1228_2112del | |
| 38 | human | 54840 | APTX | aprataxin | NR_160925.1 | 23.8% | 1_728del;1233_2117del | |
| 39 | human | 54840 | APTX | aprataxin | NR_160920.1 | 23.7% | (many diffs) | |
| 40 | human | 54840 | APTX | aprataxin | NR_160929.1 | 23% | (many diffs) | |
| 41 | human | 54840 | APTX | aprataxin | XR_929276.3 | 21.2% | 1_601del;849_1220del;1478_2368del | |
| 42 | human | 54840 | APTX | aprataxin | XR_001746326.2 | 21.2% | 1_609del;857_1228del;1486_2374del | |
| 43 | human | 54840 | APTX | aprataxin | XR_929277.3 | 21% | 1_601del;849_1243del;1501_2391del | |
| 44 | human | 54840 | APTX | aprataxin | NR_160927.1 | 20.8% | (many diffs) | |
| 45 | human | 54840 | APTX | aprataxin | XR_001746325.2 | 20.7% | (many diffs) | |
| 46 | mouse | 66408 | Aptx | aprataxin | NM_001025445.2 | 53.7% | 55.4% | (many diffs) |
| 47 | mouse | 66408 | Aptx | aprataxin | XM_006538171.3 | 53.7% | 55.4% | (many diffs) |
| 48 | mouse | 66408 | Aptx | aprataxin | NM_001025444.3 | 44.3% | 45.6% | (many diffs) |
| 49 | mouse | 66408 | Aptx | aprataxin | XM_006538166.3 | 44.3% | 45.6% | (many diffs) |
| 50 | mouse | 66408 | Aptx | aprataxin | XM_006538167.3 | 44.3% | 45.6% | (many diffs) |
| 51 | mouse | 66408 | Aptx | aprataxin | XM_006538168.3 | 44.3% | 45.6% | (many diffs) |
| 52 | mouse | 66408 | Aptx | aprataxin | XM_006538169.3 | 44.3% | 45.6% | (many diffs) |
| 53 | mouse | 66408 | Aptx | aprataxin | XM_006538170.3 | 44.3% | 45.6% | (many diffs) |
| 54 | mouse | 66408 | Aptx | aprataxin | XM_011250084.2 | 43.7% | 45% | (many diffs) |
| 55 | mouse | 66408 | Aptx | aprataxin | NM_025545.4 | 43.4% | 44.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 570
- ORF length:
- 504
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggaccccaaa atgcaggttt acaaagatga gcaggtggtg gtgataaagg 121 ataaataccc aaaggcccgt taccattggc tggtcttacc gtggacctcc atttccagtc 181 TGAAGGCTGT GGCCAGGGAA CACCTTGAAC TCCTTAAGCA TATGCACACT GTGGGGGAAA 241 AGGTGATTGT AGATTTTGCT GGGTCCAGCA AACTCCGCTT CCGATTGGGC TACCACGCCA 301 TTCCGAGTAT GAGCCATGTA CATCTTCATG TGATCAGCCA GGATTTTGAT TCTCCTTGCC 361 TTAAAAACAA AAAACATTGG AATTCTTTCA ATACAGAATA CTTCCTAGAA TCACAAGCTG 421 TGATCGAGAT GGTACAAGAG GCTGGTAGAG TAACTGTCCG AGATGGGATG CCTGAGCTCT 481 TGAAGCTGCC CCTTCGTTGT CATGAGTGCC AGCAGCTGCT GCCTTCCATT CCTCAGCTGA 541 AAGAACATCT CAGGAAGCAC TGGACACAGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 601 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 661 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATACTA 721 TTTGCTAATC ACCCATCGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 781 tgaaagatt