Transcript: Human NM_001369006.1

Homo sapiens aprataxin (APTX), transcript variant 27, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
APTX (54840)
Length:
2008
CDS:
326..940

Additional Resources:

NCBI RefSeq record:
NM_001369006.1
NBCI Gene record:
APTX (54840)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001369006.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000083638 GCTGGTTATACATTTCTGTTT pLKO.1 1453 3UTR 100% 4.950 6.930 N APTX n/a
2 TRCN0000083640 CGTTACCATTGGCTGGTCTTA pLKO.1 656 CDS 100% 4.950 3.960 N APTX n/a
3 TRCN0000294490 CTTGTGAACTTCTACTCATTT pLKO_005 1197 3UTR 100% 13.200 9.240 N APTX n/a
4 TRCN0000294491 TTTGATTCTCCTTGCCTTAAA pLKO_005 863 CDS 100% 13.200 9.240 N APTX n/a
5 TRCN0000294489 GAACACCTTGAACTCCTTAAG pLKO_005 716 CDS 100% 10.800 7.560 N APTX n/a
6 TRCN0000083641 CCGAGTATGAGCCATGTACAT pLKO.1 821 CDS 100% 4.950 3.465 N APTX n/a
7 TRCN0000083639 GCTGTGATCGAGATGGTACAA pLKO.1 971 3UTR 100% 4.950 3.465 N APTX n/a
8 TRCN0000175948 GTGTAACAAGGGATATGTCAA pLKO.1 211 5UTR 100% 4.950 3.465 N Aptx n/a
9 TRCN0000083642 GTGGTGGTGATAAAGGATAAA pLKO.1 623 CDS 100% 13.200 7.920 N APTX n/a
10 TRCN0000291579 GTGGTGGTGATAAAGGATAAA pLKO_005 623 CDS 100% 13.200 7.920 N APTX n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001369006.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14172 pDONR223 100% 80.3% 79.9% None 609_610insGCTGTGATC;612_613ins141 n/a
2 ccsbBroad304_14172 pLX_304 0% 80.3% 79.9% V5 609_610insGCTGTGATC;612_613ins141 n/a
3 TRCN0000491624 TCTACGATTCAACTCATTTGGCAA pLX_317 25.7% 80.3% 79.9% V5 609_610insGCTGTGATC;612_613ins141 n/a
4 ccsbBroadEn_12096 pDONR223 100% 46.4% 46% None 1_258del;609_610insGCTGTGATC;612_613ins141 n/a
5 ccsbBroad304_12096 pLX_304 0% 46.4% 46% V5 1_258del;609_610insGCTGTGATC;612_613ins141 n/a
6 TRCN0000474041 TACTATTTGCTAATCACCCATCGA pLX_317 76.3% 46.4% 46% V5 1_258del;609_610insGCTGTGATC;612_613ins141 n/a
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