Transcript: Human NM_001195250.2

Homo sapiens aprataxin (APTX), transcript variant 8, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
APTX (54840)
Length:
1815
CDS:
67..933

Additional Resources:

NCBI RefSeq record:
NM_001195250.2
NBCI Gene record:
APTX (54840)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001195250.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000083638 GCTGGTTATACATTTCTGTTT pLKO.1 1260 3UTR 100% 4.950 6.930 N APTX n/a
2 TRCN0000083640 CGTTACCATTGGCTGGTCTTA pLKO.1 499 CDS 100% 4.950 3.960 N APTX n/a
3 TRCN0000294490 CTTGTGAACTTCTACTCATTT pLKO_005 1004 3UTR 100% 13.200 9.240 N APTX n/a
4 TRCN0000294491 TTTGATTCTCCTTGCCTTAAA pLKO_005 706 CDS 100% 13.200 9.240 N APTX n/a
5 TRCN0000294489 GAACACCTTGAACTCCTTAAG pLKO_005 559 CDS 100% 10.800 7.560 N APTX n/a
6 TRCN0000083641 CCGAGTATGAGCCATGTACAT pLKO.1 664 CDS 100% 4.950 3.465 N APTX n/a
7 TRCN0000083639 GCTGTGATCGAGATGGTACAA pLKO.1 778 CDS 100% 4.950 3.465 N APTX n/a
8 TRCN0000083642 GTGGTGGTGATAAAGGATAAA pLKO.1 466 CDS 100% 13.200 7.920 N APTX n/a
9 TRCN0000291579 GTGGTGGTGATAAAGGATAAA pLKO_005 466 CDS 100% 13.200 7.920 N APTX n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001195250.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14172 pDONR223 100% 85% 84.7% None (many diffs) n/a
2 ccsbBroad304_14172 pLX_304 0% 85% 84.7% V5 (many diffs) n/a
3 TRCN0000491624 TCTACGATTCAACTCATTTGGCAA pLX_317 25.7% 85% 84.7% V5 (many diffs) n/a
4 ccsbBroadEn_12096 pDONR223 100% 58.3% 58.3% None 1_360del n/a
5 ccsbBroad304_12096 pLX_304 0% 58.3% 58.3% V5 1_360del n/a
6 TRCN0000474041 TACTATTTGCTAATCACCCATCGA pLX_317 76.3% 58.3% 58.3% V5 1_360del n/a
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